If you want to examine the results of gel electrophoresis — and who doesn’t — you need a transilluminator. These devices can be quite pricey, though, so you might want to check out [Gabriel St-Pierre’s] plans to make an affordable blue-light version. You can see a video about the device below.
Using a UV filter, an Arduino Nano, an LED strip, 3D printing, and some mechanical items, it looks like this is a very easy project if you need such a device. There are a few miscellaneous parts like a hinge and some mirror material, but nothing looks too exotic.
Continue reading “Affordable Transilluminator Helps Visualize DNA”
We all the know the basic components for building out an electronics lab: breadboards, bench power supply, a selection of components, a multimeter, and maybe an oscilloscope. But what exactly do you need when you’re setting up a biohacking lab?
That’s the question that [Justin] from The Thought Emporium is trying to answer with a series of videos where he does exactly that – build a molecular biology lab from scratch. In the current installment, [Justin] covers the basics of agarose gel electrophoresis, arguably the fundamental skill for aspiring bio-geeks. Electrophoresis is simply using an electric field to separate a population of macromolecules, like nucleic acids and proteins, based on their sizes. [Justin] covers the basics, from building a rig for running agarose gels to pouring the gels to doing the actual separation and documenting the results. Commercial grade gear for the job is priced to squeeze the most money out of a grant as possible, but his stuff is built on the cheap, from dollar-store drawer organizers and other odd bits. It all works, and it saves a ton of money that can be put into the things that make more sense to buy, like fluorescent DNA stain for visualizing the bands; we’re heartened to see that the potent carcinogen ethidium bromide that we used back in the day is no longer used for this.
We’re really intrigued with [Justin]’s bio lab buildout, and it inspires us to do the same here. This and other videos in the series, like his small incubators built on the cheap, will go a long way to helping others get into biohacking.
Continue reading “Simple, Low-Cost Rig Lets The Budding Biohacker Run DNA Gels”