[Philip Nicovich] has been building laser sequencers over at the University of New South Wales. His platform is used to sequence laser excitation on his fluorescence microscopy systems. In [Philip]’s case, these systems are used for super-resolution microscopy, that is breaking the diffraction limit allowing the imaging of structures of only a few nanometers (1 millionth of a millimeter) in size.
Using an Arduino shield he designed in Eagle, [Philip] was able to build the system for less than half the cost of a commercial platform.
The control system is build around the simple Arduino shield shown to the right, which uses simple 74 series logic to send TTL control signals to the laser diodes used in his rig. The Arduino runs code which allows laser firing sequences to be programmed and executed.
[Philip] also provides scripts which show how the Arduino can be interfaced with the open source micro manager control software.
As well as the schematics [Philip] has provided STEP files and drawings for the enclosure and mounts used in the system and a detailed BOM.
More useful than all this perhaps is the comprehensive write-up he provides. This describes the motivation for decisions such as the use of aluminum over steel due to its ability to transfer heat more effectively, and not to use thermal paste due to out-gassing.
While I can almost hear the cries of “not a hack”, the growing use of open source platforms and tool in academia fills us with joy. Thanks for the write-up [Philip] we look forward to hearing more about your laser systems in the future!
