Optical microscopy is over 400 years old, and in that time, it has come a long way. There are many variations of microscopes both in the selection of lenses, lighting, and other tricks to allow an instrument to coax out more information about a sample.
One proven way to increase the resolving power of a microscope is oil immersion. The sample and the lens are placed in oil that is transparent and has a high refractive index. This prevents light from refracting at the air-coverslip interface, improving the microscope’s overall performance.
The University of New South Wales has a lab that uses such a microscope. They use a special (and expensive) chamber to hold down the glass coverslip and contain the oil. The problem? At nearly $400 a pop, the chambers are a constant expense to replace, and they are not flexible enough to handle custom size requirements.
[Ben Goodnow], a first year student at the university, applied his 3D printing and laser cutting know-how to design and build a suitable chamber that costs much less and can be adapted to different projects. In addition to all the design files on GitHub, there’s also a document (PDF) that describes the design iterations and the total cost savings.
The main body uses ABS plastic, and the laser cutter produces silicone gaskets. Rare earth magnets keep the whole assembly together.
We predict a bright future for [Ben]. While you might not need an oil immersion microscope chamber, the process he followed, and the justification data he gathered are a good model for anyone who wants to justify the cost of a 3D printer and laser cutter to a boss (or, perhaps, a spouse).
Most of the microscopy on Hackaday is either aimed at SMD soldering or exotic non-optical instruments. If you want to see a comparison of oil immersion versus dry microscopy, [Bill Porter] has a video that shows the difference, below.