In the two months since the harsh realities of SARS-CoV-2 and COVID-19 have come into sharp focus, Americans have become increasingly familiar with a man who has been quietly serving the people since the days when Ronald Reagan was up for re-election. For many, Dr. Anthony Fauci is the national voice of reason in a sea of dubious information. He has arguably become the most trustworthy person the government has to offer in the face of this pandemic.
Officially, Dr. Fauci is the Director of the National Institute of Allergy and Infectious Diseases (NIAID), a position he was appointed to in 1984. He has worked under six presidents, advising them on every outbreak from the HIV/AIDS epidemic up through Zika and Ebola. Now, he is part of the White House’s coronavirus task force.
At 79 years old, he still works 18-hour days, sticking it to infectious diseases with one hand, and smoothing the feathers of the American people with the other. Dr. Fauci certainly feels like the right person at the right time. So how did he get to this point?
It was early 1983 and Françoise Barré-Sinoussi of the prestigious Pasteur Institute in Paris was busy at the centrifuge trying to detect the presence of a retrovirus. The sample in the centrifuge came from an AIDS patient, though the disease wasn’t called AIDS yet.
Just two years earlier in the US, a cluster of young men had been reported as suffering from unusual infections and forms of cancer normally experienced by the very old or by people using drugs designed to suppress the immune system. More cases were reported and US Centers for Disease Control and Prevention (CDC) formed a task force to monitor the unusual outbreak. In December, the first scientific article about the outbreak was published in the New England Journal of Medicine.
By May 1983, researchers Barré-Sinoussi and Luc Montagnier of the Pasteur Institute had isolated HIV, the virus which causes AIDS, and reported it in the journal Science. Both received the Nobel prize in 2008 for this work and the Nobel prize citation stated:
Never before have science and medicine been so quick to discover, identify the origin and provide treatment for a new disease entity.
It’s only fitting then that we take a closer look at one of these modern detectives of science, Françoise Barré-Sinoussi, and what led to her discovery.
When we think of machine learning it’s usually in the context of robotics—giving an algorithm a large set of input data in order to train it for a certain task like navigation or understanding your handwriting. But it turns out you can also train a nasty virus to go to sleep and never wake up again. That’s exactly what the Immunity Project has been doing. They believe that they have a viable HIV vaccine and are trying to raise about $25 million to begin human testing.
The vaccine hacks the Human Immunodeficiency Virus itself, forcing it to mutate into a dormant form that will not attack its human carrier. It sounds so simple, but a lot of existing knowledge and procedures, as well as new technology, went into getting this far. Last week we spoke with [Reid Rubsamen, M.D.] about the process, which began by collecting blood samples from a wide range of “Controllers“. Controllers are people who carry HIV but manage to suppress the virus’s progression to AIDS. How do you find these people? That’s another story which Scientific American covered (PDF); the short answer is that thanks to the work of [Bruce D. Walker, M.D.] there was already a database of Controllers available.
The information accumulated by [Walker] then underwent a data crunching exercise. The data set was so enormous that a novel approach was adopted. For the laymen this is described as a spam filter: using computers to look at large sets of email to develop a complex process for sifting real messages out of the noise. The task at hand is to look at the genotype of a Controller and compare it with the epitope— a short chain of proteins—in the virus they carry. The power of machine learning managed to whittle down all the data to a list of the first six epitopes that have the desired dormant-mutation property. The vaccine consists of a cocktail of these epitopes. It does, however, require some clever delivery tactics to reach the parts of the world where it’s most needed. The vaccine must not require refrigeration nor any special skills to administer.
The vaccine’s production uses existing methods to synthesize the amino acid peptides, which are the epitopes themselves. The packaging, however, is a new concept. [Dr. Rubsamen’s] company, Flow Parma, Inc., is using microspheres to encapsulate the vaccine, which render it shelf-stable and allow it to be administered through a nasal spray. Learn more about the technology behind the production of microspheres from this white paper (PDF).
If the vaccine (which will be produced without profit) passes clinical trials, it could see mass distribution as early as 2017.