Pong In A Petri Dish: Teasing Out How Brains Work

September 14, 2024 by Maya Posch 1 Comment

Experimental setup for the EAP hydrogel free energy principle test. (Credit: Vincent Strong et al., Cell, 2024)

Of the many big, unanswered questions in this Universe, the ones pertaining to the functioning of biological neural networks are probably among the most intriguing. From the lowliest neurally gifted creatures to us brainy mammals, neural networks allow us to learn, to predict and adapt to our environments, and sometimes even stand still and wonder puzzlingly how all of this even works. Such puzzling has led to a number of theories, with a team of researchers recently investigating one such theory, as published in Cell. The focus here was that of Bayesian approaches to brain function, specifically the free energy principle, which postulates that neural networks as inference engines seek to minimize the difference between inputs (i.e. the model of the world as perceived) and its internal model.

This is where Electro Active Polymer (EAP) hydrogel comes into play, as it features free ions that can migrate through the hydrogel in response to inputs. In the experiment, these inputs are related to the ball position in the game of Pong. Much like experiments involving biological neurons, the hydrogel is stimulated via electrodes (in a 2 x 3 grid, matching the 2 by 3 grid of the game world), with other electrodes serving as outputs. The idea is that over time the hydrogel will ‘learn’ to optimize the outputs through ion migration, so that it ‘plays’ the game better, which should be reflected in the scores (i.e. the rally length).

Based on the results some improvement in rally length can be observed, which the researchers present as statistically significant. This would imply that the hydrogel displays active inference and memory. Additional tests with incorrect inputs resulted in a marked decrease in performance. This raises many questions about whether this truly displays emergent memory, and whether this validates the free energy principle as a Bayesian approach to understanding biological neural networks.

To the average Star Trek enthusiast the concept of hydrogels, plasmas, etc. displaying the inklings of intelligent life would probably seem familiar, and for good reason. At this point, we do not have a complete understanding of the operation of the many billions of neurons in our own brains. Doing a bit of prodding and poking at some hydrogel and similar substances in a dish might be just the kind of thing we need to get some fundamental answers.

How Photomultipliers Detect Single Photons

September 12, 2024 by Maya Posch 12 Comments

If you need to measure the presence of photons down to a very small number of them, you are looking at the use of a photomultiplier, as explained in a recent video by [Huygens Optics] on YouTube. The only way to realistically measure at such a sensitivity level is to amplify them with a photomultiplier tube (PMT). Although solid-state alternatives exist, this is still a field where vacuum tube-based technology is highly relevant.

Despite being called ‘photomultipliers’, these PMTs actually amplify an incoming current (electron) in a series of dynode stages, to create an output current that is actually easy to quantify for measurement equipment. They find uses in everything from Raman spectroscopy to medical diagnostics and night vision sensors.

The specific PMT that [Huygens Optics] uses in the video is the Hamamatsu R928. This has a spectral response from 185 nm to 900 nm. The electrode mesh is where photons enter the tube, triggering the photo cathode which then ejects electrons. These initial electrons are then captured and amplified by each dynode stage, until the anode grid captures most of the electrons. The R928 has a gain of 1.0 x 10⁷ (10 million) at -1 kV supply voltage, so each dynode multiplies the amount of electrons by six, with a response time of 22 ns.

PMTs are unsurprisingly not cheap, but [Huygens Optics] was lucky to find surplus R928s on Marktplaats (Dutch online marketplace) for €100 including a cover, optics and a PCB with the socket, high-voltage supply (Hamamatsu C4900) and so on. Without documentation the trick was to reverse-engineer the PCB’s connections to be able to use it. In the video the components and their function are all briefly covered, as well as the use of opamps like the AD817 to handle the output signal of the R928. Afterwards the operation of the PMT is demonstrated, which makes clear just how sensitive the PMT is as it requires an extremely dark space to not get swamped with photons.

An interesting part about the demonstration is that it also shows the presence of thermionic emissions: anode dark current in the datasheet. This phenomenon is countered by cooling the PMT to prevent these emissions if it is an issue. In an upcoming video the R928 will be used for more in-depth experiments, to show much more of what these devices are capable of.

Thanks to [cliff claven] for the tip.

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Shedding New Light On The Voynich Manuscript With Multispectral Imaging

September 10, 2024 by Donald Papp 14 Comments

The Voynich Manuscript is a medieval codex written in an unknown alphabet and is replete with fantastic illustrations as unusual and bizarre as they are esoteric. It has captured interest for hundreds of years, and expert [Lisa Fagin Davis] shared interesting results from using multispectral imaging on some pages of this highly unusual document.

We should make it clear up front that the imaging results have not yielded a decryption key (nor a secret map or anything of the sort) but the detailed write-up and freely-downloadable imaging results are fascinating reading for anyone interested in either the manuscript itself, or just how exactly multispectral imaging is applied to rare documents. Modern imaging techniques might get leveraged into things like authenticating sealed packs of Pokémon cards, but that’s not all it can do.

Because multispectral imaging involves things outside our normal perception, the results require careful analysis rather than intuitive interpretation. Here is one example: multispectral imaging may yield faded text visible “between the lines” of other text and invite leaping to conclusions about hidden or erased content. But the faded text could be the result of show-through (content from the opposite side of the page is being picked up) or an offset (when a page picks up ink and pigment from its opposing page after being closed for centuries.)

[Lisa] provides a highly detailed analysis of specific pages, and explains the kind of historical context and evidence this approach yields. Make some time to give it a read if you’re at all interested, we promise it’s worth your while.

The Science Of Coating Steel

September 9, 2024 by Al Williams 6 Comments

[Breaking Taps] has a look at “parkerization” — a process to coat steel to prevent rust. While you commonly see this finish in firearms, it is usable anywhere you need some protection for steel parts. The process is relatively easy. It does require heat and a special manganese solution made for the purpose. You scuff up the surface of the steel and degrease and wash it.

Once the part is ready, you insert the part in hot solution which is manganese and phosphoric acid. Rinse and displace the water and you are ready to oil the part.

But what we really liked was the electron micrographs of the steel before and after the process. The phosphates formed in the solution cover the iron and hold oil to prevent oxidization. However, the first attempt wasn’t uniform so it wouldn’t work as well. [Breaking Taps] thinks it was a failure to rough up the piece sufficiently before starting. He also raised the temperature of the bath and got a better, but not perfect, result.

We miss having an electron microscope at work and we really want one at home! The last fun coating project we remember used copper in a strange and wonderful way.

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Rendering Skin Transparent Using The Food Dye Tartrazine

September 7, 2024 by Maya Posch 21 Comments

Although we generally assume that opacity is the normal look for animals like us humans, this factoid is only correct for as long as you maintain the dissimilar optical refraction indices of skin and the more aqueous underlying structures. What if you could change the refraction index of skin? If you could prevent the normal scattering at the interface, you could reveal the structures underneath, effectively rendering skin transparent. [Zihao Uo] and others demonstrate this in a paper published in Science.

The substance they used was the common food dye known as tartrazine, which also goes by the names of Yellow 5 and E102 when it is used in food (like Doritos), cosmetics, and drugs. By rubbing the tartrazine into the skin of mice, the researchers were able to observe underlying blood vessels and muscles. Simulations predicted that the dye would change the refraction index mismatch between lipids and water which normally causes the light scattering that creates the skin’s opaque appearance. With the dye rubbed into the skin, the effect worked to a depth of about 3 mm, which makes it useful for some research and possible medical applications, but not quite at the ‘jellyfish-transparency’ levels that some seem to have imagined at the news.

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Launching Model Airplanes With A Custom Linear Induction Motor

September 5, 2024 by Maya Posch 20 Comments

Launching things with electromagnetism is pretty fun, with linear induction motors being a popular design that finds use from everywhere in hobby designs like [Tom Stanton]’s to the electromagnetic launchers on new US and Chinese aircraft carriers. Although the exact design details differ, they use magnetic attraction and repulsion to create a linear motion on the propulsive element, like the sled in [Tom]’s design. Much like the electromagnetic catapults on a Gerald R. Ford-class carrier, electrical power is applied to rapidly move the sled through the channel, akin to a steam piston with a steam catapult.

Model airplane sparking its way through the launcher’s channel. (Credit: Tom Stanton, YouTube)

For [Tom]’s design, permanent magnets are used along both sides of the channel in an alternating north/south pole fashion, with the sled using a single wound coil that uses brushes to contact metal rails along both sides of the channel. Alternating current is then applied to this system, causing the coil to become an electromagnet and propel itself along the channel.

An important consideration here is the number of turns of wire on the sled’s coil, as this controls the current being passed, which is around 90 A for 100 turns. Even so, the fastest sled design only reached a speed of 44 mph (~71 km/h), which is 4 mph faster than [Tom]’s previous design that used coils alongside the channels and a sled featuring a permanent magnet.

One way to increase the speed is to use more coils on the sled, with a two-coil model launching a light-weight model airplane to 10.2 m/s, which is not only a pretty cool way to launch an airplane, but also gives you a sense of appreciation for the engineering challenges involved in making an electromagnetic catapult system work for life-sized airplanes as they’re yeeted off an aircraft carrier and preferably not straight into the drink.

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Spiders Are Somehow Hacking Fireflies To Lure More Victims

September 2, 2024 by Donald Papp 27 Comments

What happens when an unfortunate bug ends up in a spider’s web? It gets bitten and wrapped in silk, and becomes a meal. But if the web belongs to an orb-weaver and the bug is a male firefly, it seems the trapped firefly — once bitten — ends up imitating a female’s flash pattern and luring other males to their doom.

Fireflies communicate with flash patterns (something you can experiment with yourself using nothing more than a green LED) and males looking to mate will fly around flashing a multi-pulse pattern with their two light-emitting lanterns. Females will tend to remain in one place and flash single-pulse patterns on their one lantern.

When a male spots a female, they swoop in to mate. Spiders have somehow figured out a way to actively take advantage of this, not just inserting themselves into the process but actively and masterfully manipulating male fireflies, causing them to behave in a way they would normally never do. All with the purpose of subverting firefly behavior for their own benefit.

It all started with an observation that almost all fireflies in webs were male, and careful investigation revealed it’s not just some odd coincidence. When spiders are not present, the male fireflies don’t act any differently. When a spider is present and detects a male firefly, the spider wraps and bites the firefly differently than other insects. It’s unknown exactly what happens, but this somehow results in the male firefly imitating a female’s flash patterns. Males see this and swoop in to mate, but with a rather different outcome than expected.

The research paper contains added details but it’s clear that there is more going on in this process than meets the eye. Spiders are already fascinating creatures (we’ve seen an amazing eye-tracking experiment on jumping spiders) and it’s remarkable to see this sort of bio-hacking going on under our very noses.