Microfluidic LEGO Bricks

Years ago, prototyping microfluidic systems was a long, time-intensive task. With inspiration from DIY PCB fabrication techniques, that time is now greatly reduced. However, even with the improvements, it still takes a full day to go from an idea to a tangible implementation. However, progress creeps in this petty pace from day to day, and in accordance, a group of researchers have found a way to use 3D printed molds to create microfluidic LEGO bricks that make microfluidic prototyping child’s play.

For the uninitiated, microfluidics is the study and manipulation of very small volumes of water, usually a millionth of a liter and smaller (nL-pL). Interestingly, the behavior of fluids at small scales differs greatly from its larger scale brethren in many key ways. This difference is due to the larger role surface tension, energy dissipation, and fluidic resistance play when distances and volumes are minimized.

By using 3D printed molds to create microfluidic bricks that fit together like LEGOs, the researchers hope to facilitate medical research. Even though much research relies on precise manipulation of minuscule amounts of liquid, most researchers pipette by hand (or occasionally by robot), introducing a high level of human error. Additionally, rather than needing multiple expensive micropipettes, a DIY biohacker only needs PDMS (a silicon-based chemical already used microfluidics) and 3D printed molds to get started in prototyping biological circuits. However, if you prefer a more, ahem, fluid solution, we’ve got you covered.

[via Adafruit]

Microfluidics “Frogger” Is A Game Changer For DIY Biology

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See those blue and green dots in the GIF? Those aren’t pixels on an LCD display. Those are actual drops of liquid moving across a special PCB. The fact that the droplets are being manipulated to play a microfluidics game of “Frogger” only makes OpenDrop v 2.0 even cooler.

Lab biology is mainly an exercise in liquid handling – transferring a little of solution X into some of solution Y with a pipette. Manual pipetting is tedious, error prone, and very low throughput, but automated liquid handling workstations run into the hundreds of thousands of dollars. This makes [Urs Gaudenz]’s “OpenDrop” microfluidics project a potential game changer for the nascent biohacking movement by offering cheap and easy desktop liquid handling.

Details are scarce on the OpenDrop website as to exactly how this works, but diving into the literature cited reveals that the pads on the PCB are driven to high voltages to attract the droplets. The PCB itself is covered with a hydrophobic film – Saran wrap that has been treated with either peanut oil or Rain-X. Moving the droplets is a simple matter of controlling which pads are charged. Splitting drops is possible, as is combining them – witness the “frog” getting run over by the blue car.

There is a lot of cool work being done in microfluidics, and we’re looking forward to see what comes out of this open effort. We’ve covered other open source efforts in microfluidics before, but this one seems so approachable that it’s sure to capture someone’s imagination.

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Open Microfluidics Instrumentation Playset

Micro-what? Microfluidics! It’s the field of dealing with tiny, tiny bits of fluids, and there are some very interesting applications in engineering, biology, and chemistry. [Martin Fischlechner], [Jonathan West], and [Klaus-Peter Zauner] are academic scientists who were working on microfluidics and made their own apparatus, initially because money was tight. Now they’ve stuck to the DIY approach because they can get custom machinery that simply doesn’t exist.

In addition to their collaboration, and to spread the ideas to other labs, they formed DropletKitchen to help advance the state of the art. And you, budding DIY biohacker, can reap the rewards.

In particular, the group is focused on droplet microfluidics. Keeping a biological or chemical reaction confined to its own tiny droplet is like running it inside its own test-tube, but because of the high rate at which the droplets can be pumped out, literally millions of these test-tubes are available. Want to grow hundreds of thousands of single cells, each in their own environment? Done.

The DropletKitchen kit includes an accurate pump system, along with high-speed camera and flash setups to verify that everything’s working as it should. Everything is open-source, and a lot of it is 3D-printable and written in OpenSCAD so that it’s even easy to modify to fit your exact needs. You just need to bring the science.

This is a professional-grade open source project, and we’re excited to see it when academics take a turn toward the open. Bringing cutting edge processing technologies within reach of the biohacker community is a huge multiplier. We can’t wait to see what comes out of this.