It sounds like an overly complicated method a supervillain would use to slowly and painfully eliminate enemies — a chamber with variable oxygen concentration. This automated environmental chamber isn’t for torturing suave MI6 agents, though; rather, it enables cancer research more-or-less on the cheap.
Tasked with building something to let his lab simulate the variable oxygen microenvironments found in some kinds of tumors, [RyanM415] first chose a standard lab incubator as a chamber to mix room air with bottled nitrogen. With a requirement to quickly vary the oxygen concentration from the normal 21% down to zero, he found that the large incubator took far too long to equilibrate, and so he switched to a small acrylic box. Equipped with a mixing fan, the smaller chamber quickly adjusts to setpoints, with an oxygen sensor providing feedback and controlling the gas valves via a pair of Arduinos. It’s quite a contraption, with floating ball flowmeters and stepper-actuated variable gas valves, but the results are impressive. If it weren’t for the $2000 oxygen sensor, [RyanM145] would have brought the whole project in for $500, but at least the lab can use the sensor elsewhere.
Modern biology and chemistry labs are target-rich environments for hacked instrumentation. From DIY incubators to cheap electrophoresis rigs, we’ve got you covered.
Continue reading “Automated Chamber Passes Just the Right Gas”
When we think of role models, it’s easy to categorize them narrowly on the basis of their skill set. We might say that he’s a great mathematician, or that she is an excellent chemist. Some role models are admirable on a deeper, human level. These are the kinds of heroes who obliterate all the obstacles dropped in front of them to tirelessly pursue their interests and devote their lives to doing the kind of stuff that makes the world better for everyone.
Italian Nobel Laureate Rita Levi-Montalcini is this kind of role model. Her scientific curiosity and unconventional thinking led her to discover nerve growth factor (NGF), a naturally occurring protein which we now know is responsible for nerve growth and regulation. Rita’s discovery provided great insight into the way the nervous system develops. The discoveries that she made underlie much of modern research into neurologically degenerative diseases like Alzheimer’s and cancer, and NGF is used experimentally the treatment of both.
Continue reading “Rita Levi-Montalcini Had Nerves of Steel”
[Michael Balzer] shows us that you are your own best advocate when it comes to medical care – having the ability to print models of your own tumors is a bonus. [Michael’s] wife, Pamela, had been recovering from a thyroidectomy when she started getting headaches. She sought a second opinion after the first radiologist dismissed the MRI scans of her head – and learned she had a 3 cm tumor, a meningioma, behind her left eye. [Michael], host of All Things 3D, asked for the DICOM files (standard medical image format) from her MRI. When Pamela went for a follow-up, it looked like the tumor had grown aggressively; this was a false alarm. When [Michael] compared the two sets of DICOM images in Photoshop, the second MRI did not truly show the tumor had grown. It had only looked that way because the radiologist had taken the scan at a different angle! Needless to say, the couple was not pleased with this misdiagnosis.
However, the meningioma was still causing serious problems for Pamela. She was at risk of losing her sight, so she started researching the surgery required to remove the tumor. The most common surgery is a craniotomy: the skull is sawed open and the brain physically lifted in order to access the tumor below it. Not surprisingly, this carries a high risk of permanent damage to any nerves leading to loss of smell, taste, or sight if the brain is moved the wrong way. Pamela decided to look for an alternative surgery that was less invasive. [Michael] created a 3D print of her skull and meningioma from her MRIs. He used InVesalius, free software designed to convert the 2D DICOM files into 3D images. He then uploaded the 3D rendered skull to Sketchfab, sharing it with potential neurologists. Once a neurologist was found that was willing to consider an alternative surgery, [Michael] printed the skull and sent it to the doctor. The print was integral in planning out the novel procedure, in which a micro drill was inserted through the left eyelid to access the tumor. In the end, 95% of the tumor was removed with minimal scarring, and her eyesight was spared.
If you want to print your own MRI or CT scans, whether for medical use or to make a cool mug with your own mug, there are quite a few programs out there that can help. Besides the aforementioned InVesalius, there is DeVIDE, Seg3D, ImageVis3D, and MeshLab or MeshMixer.