We are normally told that microwave ovens are strictly for food only, and that anything else will cause all sorts of bad things to come our way. There can be few readers who haven’t at some time seen the shower of sparks when an inadvertent metallic object finds its way onto the turntable.
A particularly useful non-food application for a microwave oven comes in the form of the small kilns sold for glass fusing. These are ceramic cylinders coated internally with silicon carbide, and [ShakeTheFuture] shows us how to make your own.
Key to the process is ceramic fibre insulation, which is bonded both to itself and to the fused silicon carbide grit by a cured solution of waterglass, sodium silicate. The result can easily reach the required temperature for fusing glass, but also has an application in burning out surplus wax or PLA from a plaster mould. It’s particularly interesting to see the technique with the waterglass in action, and you can see a run-down of the whole thing in the video below the break.
Continue reading “A Microwave Kiln, From Scratch”
When you consider that almost every single cell in your body has more than a meter of DNA coiled up inside its nucleus, it seems like it should be pretty easy to get some to study. But with all the other cellular gunk in a crude preparation, DNA can be quite hard to isolate. That’s where this cheap and easy magnetic DNA separation method comes in. If it can be optimized and tested with some help from the citizen science community.
Commercial DNA separation methods generally involve mixing silica beads into crude cell fractions; the DNA preferentially binds to the silica, making it possible to mechanically separate it from the rest of the cellular junk. But rather than using a centrifuge to isolate the DNA, [Justin] from The Thought Emporium figured that magnets might do a better job. It’s not a new idea — biotech companies offer magnetic separation beads commercially, but at too steep a price for [Justin]’s budget. His hack comes from making magnetite particles from common iron compounds like PCB etchant and moss killer, and household ammonia cleaner. The magnetite particles are then coated with sodium silicate solution, also known as waterglass. The silica coating should allow the beads to bind to DNA, with the magnetic core taking care of separation.
[Justin] was in the process of testing his method when he lost access to the needed instruments, so he’s appealing to the larger science community for help optimizing his technique. Based on his track record of success in fields ranging from satellite tracking to graphene production, we’ll bet he’ll nail this one too.
Continue reading “Cheap And Easy Magnetic DNA Separation Method Needs Your Help”