Cheap and Easy Magnetic DNA Separation Method Needs Your Help

When you consider that almost every single cell in your body has more than a meter of DNA coiled up inside its nucleus, it seems like it should be pretty easy to get some to study. But with all the other cellular gunk in a crude preparation, DNA can be quite hard to isolate. That’s where this cheap and easy magnetic DNA separation method comes in. If it can be optimized and tested with some help from the citizen science community.

Commercial DNA separation methods generally involve mixing silica beads into crude cell fractions; the DNA preferentially binds to the silica, making it possible to mechanically separate it from the rest of the cellular junk. But rather than using a centrifuge to isolate the DNA, [Justin] from The Thought Emporium figured that magnets might do a better job. It’s not a new idea — biotech companies offer magnetic separation beads commercially, but at too steep a price for [Justin]’s budget. His hack comes from making magnetite particles from common iron compounds like PCB etchant and moss killer, and household ammonia cleaner. The magnetite particles are then coated with sodium silicate solution, also known as waterglass. The silica coating should allow the beads to bind to DNA, with the magnetic core taking care of separation.

[Justin] was in the process of testing his method when he lost access to the needed instruments, so he’s appealing to the larger science community for help optimizing his technique. Based on his track record of success in fields ranging from satellite tracking to graphene production, we’ll bet he’ll nail this one too.

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Graphene from Graphite by Electrochemical Exfoliation

Graphene is an interesting material, but making enough of the stuff to do something useful can be a little tough. That’s why we’re always on the lookout for new methods, like this electrochemical process for producing graphene in bulk.

You probably know that graphene is a molecular monolayer of carbon atoms linked in hexagonal arrays. Getting to that monolayer is a difficult proposition, but useful bits of graphene can be created by various mechanical and chemical treatments of common graphite. [The Thought Emporium]’s approach to harvesting graphene from graphite is a two-step process starting with electrochemical exfoliation. Strips of thin graphite foil are electrolyzed in a bath of ferrous sulfate, resulting in the graphite delaminating and flaking off into the electrolyte. After filtering and cleaning, the almost graphene is further exfoliated in an ultrasonic cleaner. The result is gram quantity yields with very little work and at low cost.

There’s plenty of effort going into new methods of creating graphene these days, whether by barely controlled explosions or superheating soybean oil. But will graphene be the Next Big Thing? The jury is still out on that.

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