Coronavirus Testing: CRISPR Technology Set To Streamline Viral Testing

May 27, 2020 by Dan Maloney 3 Comments

If we could run back 2020 to its beginning and get a do-over, chances are pretty good that we’d do a lot of things differently. There’s a ton of blame to go around on COVID-19, but it’s safe to say that one of the biggest failures of this whole episode has been the lack of cheap, quick, accurate testing for SARS-CoV-2, the virus behind the current pandemic. It’s not for lack of information; after all, Chinese scientists published the sequence of the viral genome very early in the pandemic, and researchers the world over did the same for all the information they gleaned from the virus as it rampaged around the planet.

But leveraging that information into usable diagnostics has been anything but a smooth process. Initially, the only method of detecting the virus was with reverse transcriptase-polymerase chain reaction (RT-PCR) tests, a fussy process that requires trained technicians and a well-equipped lab, takes days to weeks to return results, and can only tell if the patient has a current infection. Antibody testing has the potential for a quick and easy, no-lab-required test, but can only be used to see if a patient has had an infection at some time in the past.

What’s needed as the COVID-19 crisis continues is a test with the specificity and sensitivity of PCR combined with the rapidity and simplicity of an antibody test. That’s where a new assay, based on the latest in molecular biology methods and dubbed “STOPCovid” comes in, and it could play a major role in diagnostics now and in the future.

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Improving 3D Printed Supports With A Marker

May 27, 2020 by Tom Nardi 43 Comments

Anyone who’s spent some quality time with a desktop 3D printer is familiar with the concept of supports. If you’re working with a complex model that has overhanging features, printing a “scaffolding” of support material around it is often required. Unfortunately, supports can be a pain to remove and often leave marks on the finished print that need to be addressed.

Looking to improve the situation, [Tumblebeer] has come up with a very unique modification to the traditional approach that we think is certainly worthy of closer examination. It doesn’t remove the need for support material, but it does make it much easier to remove. The method is cheap, relatively simple to implement, and doesn’t require multiple extruders or filament switching as is the case with something like water-soluble supports.

The trick is to use a permanent marker as a release agent between the top of the support and the area of the print it’s actually touching. The coating of marker prevents the two surfaces from fusing, while still providing the physical support necessary to keep the model from sagging or collapsing.

To test this concept, [Tumblebeer] has outfitted a Prusa i3 MK3S with a solenoid actuated marker holder that hangs off the side of the extruder assembly. The coil is driven from the GPIO pins of a Raspberry Pi running OctoPrint, and is engaged by a custom command in the G-code file. It keeps the marker out of the way during normal printing, and lowers it when its time to lay down the interface coating.

[Tumblebeer] says there’s still a bit of hand-coding involved in this method, and that some automated G-code scripts or a custom slicer plugin could streamline the process considerably. We’re very interested in seeing further community development of this concept, as it seems to hold considerable promise. Having a marker strapped to the side of the extruder might seem complex, but it’s nothing compared to switching out filaments on the fly.

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Hacking Dell Laptops To Use Off-Brand Chargers

May 27, 2020 by Lewin Day 67 Comments

Dell, along with many other manufacturers, have begun to implement smart features into their laptop charging circuitry. This leaves the user out of luck if they wish to use an off-brand part, or get caught short when their original charger fails. [Neutrino] was in just such a position, and decided to hack around the problem.

The laptop verifies the identity of the attached charger by a third pin. This communicates with a One-Wire IC embedded in the charger, which reports the charger’s identity when queried by the laptop. When [Neutrino]’s charger broke, an attempt was made to use an off-brand charger, with the third pin hooked up to the original failed unit. This tricked the laptop into charging successfully.

For a more permanent workaround, [Neutrino] harvested the One-Wire IC from inside the original charger, and instead hooked it up inside the laptop, directly to the charge port. Thus, the laptop always thinks a Dell charger is connected when power is applied. There is some risk, in that if the user plugs in a lower-power charger than the original, there could be an overload event, but that’s just the risk inherent in the hack.

It’s a tidy workaround for an annoying problem that is all too common in the post-DRM world. Laptop chargers are often prime candidates for failure too; we’ve seen fixes as creative as repairing a Magsafe with a pistacchio nut before!

[Thanks to Levi for the tip]