Impossibly Huge Depth of Focus in Microscope Photographs

Sometimes, less is more. Sometimes, more is more. There is a type of person who believes that if enough photos of the same subject are taken, one of them will shine above the rest as a gleaming example of what is possible with a phone camera and a steady hand. Other people know how to frame a picture before hitting the shutter button. In some cases, the best method may be snapping a handful of photos to get one good one, not by chance, but by design.

[The Thought Emporium]’s video, also below the break, is about getting crisp pictures from a DSLR camera and a microscope using focus stacking, sometimes called image stacking. The premise is to take a series of photos that each have a different part of the subject in focus. In a microscope, this range will be microscopic but in a park, that could be several meters. When the images are combined, he uses Adobe products, the areas in focus are saved while the out-of-focus areas are discarded and the result is a single photo with an impossible depth of focus. We can’t help but remember those light-field cameras which didn’t rely on moving lenses to focus but took many photos, each at a different focal range.

[The Thought Emporium] has shown us his photography passion before, as well as his affinity for taking the cells out of plants and unusual cuts from the butcher and even taking a noble stab at beating lactose intolerance.

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DSLR trick lets you change focus after taking the picture

Here’s two photographic takes on the same subject, each with a different depth of focus. [Chaos Collective] came up with a way to make interactive still images that allow a user to adjust the depth of focus by clicking on different objects in the image.

This was inspired by the Lytro camera which uses an array of lenses to take multiple pictures at once. Each of those images has a slightly different depth of focus. The technique used here doesn’t require that you buy one of those $400+ cameras. But it’s not a cheap hack unless you already own a camera that can shoot video and has manual focus.

The technique used by the [Chaos Collective] is to move the camera’s manual focus setting from the nearest to the furthest target while capturing a video. That file can then be processed using their browser-based tool which turns it into an embedded HTML5 image.