There was a time when electronics was very much a hobby that existed in the macroscopic world. Vacuum tubes, wire-wound resistors, and big capacitors were all mounted on terminal strips and mounted in a heavy chassis or enclosure, and interfacing with everything from components to tools was more an exercise in gross motor skills than fine. Even as we started to shrink components down to silicon chips, the packages we put them in were still large enough to handle and see easily. It’s only comparatively recently that everything has started to push the ludicrous end of the scale, with components and processes suitable only for microscopic manipulation, but that’s pretty much where we are now, and things are only likely to get smaller as time goes on.
Zach is pretty comfortable working in and around the microscopic realm, and he’ll stop by the Hack Chat to share what he’s been up to down there. We’ll talk about all the cool stuff going on in Zach’s lab, and see what else he has in store for us.
Our Hack Chats are live community events in the Hackaday.io Hack Chat group messaging. This week we’ll be sitting down on Wednesday, June 23 at 12:00 PM Pacific time. If time zones have you tied up, we have a handy time zone converter.
Click that speech bubble to the right, and you’ll be taken directly to the Hack Chat group on Hackaday.io. You don’t have to wait until Wednesday; join whenever you want and you can see what the community is talking about.
For anyone that’s fiddled around with a magnifying glass, it’s pretty easy to understand how optical microscopes work. And as microscopes are just an elaboration on a simple hand lens, so too are electron microscopes an elaboration on the optical kind, with electrons and magnets standing in for light and lenses. But atomic force microscopes? Now those take a little effort to wrap your brain around.
Luckily for us, [Zachary Tong] over at the Breaking Taps YouTube channel recently got his hands on a remarkably compact atomic force microscope, which led to this video about how AFM works. Before diving into the commercial unit — but not before sharing some eye-candy shots of what it can do — [Zach] helpfully goes through AFM basics with what amounts to a macro version of the instrument.
His macro-AFM uses an old 3D-printer as an X-Y-Z gantry, with a probe head added to the printer’s extruder. The probe is simply a sharp stylus on the end of a springy armature, which is excited into up-and-down oscillation by a voice coil and a magnet. The probe rasters over a sample — he looked at his 3D-printed lattices — while bouncing up and down over the surface features. A current induced in the voice coil by the armature produces a signal that’s proportional to how far the probe traveled to reach the surface, allowing him to map the sample’s features.
The actual AFM does basically the same thing, albeit at a much finer scale. The probe is a MEMS device attached to — and dwarfed by — a piece of PCB. [Zach] used the device to image a range of samples, all of which revealed fascinating details about the nanoscale realm. The scans are beautiful, to be sure, but we really appreciated the clear and accessible explanation of AFM.
The technique also requires image processing. Light passing through the metamaterial breaks into speckles that produce low-resolution images that can combine to form high-resolution images. This so-called structured illumination technique isn’t exactly new, but previous techniques allowed about 100-nanometer resolution, much less than what the researchers were able to find using this material.
Being a bit shocked at the prices of articulating arm microscope mounts, not to mention the shipping fees to the UK, [CapTec] realized they looked substantially similar to your typical computer monitor arm mount. Thinking he could adapt a monitor arm for much less money, he fired up FreeCAD and started designing.
[CapTec] is using this to support his Amscope / Eakins camera-equipped trinocular microscope, but notes that the same mechanical bracket / focus rack interface is found on binocular ‘scopes as well. He observes that the mount is no more stable than your desk or lab bench, so keep that in mind.
Ultimately the monitor arm set him back less than $40, and all told he reckons the whole thing was under $55. Based on prices he’s been researching online, this represents a savings of well over $200. In his calculations, the shipping fee comprised quite a hefty percentage of the total cost. We wonder if they are artificially high due to coronavirus — if so, the make / buy price comparison might yield different results in the future.
This type of project is a perfect use-case for a home 3D printer — making your own parts when the normal supply channels are unavailable or overpriced. Are articulating arms that are purpose-built for microscopes significantly different than those designed for big computer monitors? If you know, please comment down below.
When one thinks about microscopy, it seems to be mostly qualitative. Looking at a slide teeming with bacteria or protozoans is less about making measurements and more about recognizing features and describing their appearance. Not all microscopes are created equal, though, with some being far more optimized for making fine measurements of the microscopic realm.
This 3D-printed confocal laser scanning microscope is a good example of an instrument for measuring really small stuff. As [Zachary Tong] points out, confocal scanning microscopy uses a clever optical setup to collect light from a single, well-defined point within a sample; rather than getting an image of all the points within a two-dimensional focal plane, the scanning function moves the focal point around through the sample in three dimensions, capturing spatial data to go along with the optical information.
The stage of [Zach]’s microscope is based on OpenFlexure’s Delta Stage, an open-source, 3D-printed delta-bot motion control platform that’s capable of positioning samples with sub-micron precision. Above the stage are the deceptively simple optics, with a laser diode light source, an objective lens, and a photodiode detector behind a pinhole. The detector feeds a homebrew trans-impedance amplifier that captures data at millions of points as the sample is moved through a small three-dimensional space. All that data gets crunched to find the Z-axis position corresponding to the maximum intensity at each point.
It takes a while to gather all this data — up to several days for even a small sample — but it works pretty well. [Zach] already has some ideas for reducing noise and speeding up the scan time; perhaps a stage based on DVD parts like this one would be faster than the delta stage. We look forward to seeing his improvements.
The microscope build actually took two forms. One, a regular digital microscope any of us may be familiar with, using a C-mount microscope lens fitted to a Raspberry Pi HQ camera. The other, a polarimetric microscope, using an Allied Vision Mako G-508B POL polarimetric camera instead, with the same microscope lens. The polarimetric camera takes stunning false-color images, where the color values correspond to the polarization of the light bouncing off an object. It’s incredibly specialized hardware with a matching price tag, but [E/S Pronk] hopes to build a cheaper DIY version down the line, too.
One aspect of working for Hackaday comes in our regular need to take good quality photographs for publication. I have a semi-decent camera that turns my inept pointing and shooting into passably good images, but sometimes the easiest and quickest way to capture something is to pull out my mobile phone.
It’s a risky step because phone camera modules and lenses are tiny compared to their higher quality cousins, and sometimes the picture that looks good on the phone screen can look awful in a web browser. You quickly learn never to zoom on a mobile phone camera because it’s inevitably a digital zoom that simply delivers grainy interpolated pictures.
That’s not to say that the zoom can’t be useful. Recently I had some unexpected inspiration when using a smartphone camera as a magnifier to read the writing on a chip. I don’t need an archival copy of the image… I just needed a quick magnifying tool. Have I been carrying a capable magnifier for soldering in my pocket or handbag for years without realising it? I decided to give it a try and it worked okay with a few caveats. While I have seen optics turn these cameras into pretty good microscopes, my setup added nothing more than a phone tripod, and will get you by in a pinch.