A researcher in a safety harness pollinates an American chestnut tree from a lift. Another researcher is on the other side of the lift and appears to be taking notes. The tree has bags over some of its branches, presumably to control the pollen that gets in. The lift has a grey platform and orange arm.

Hacking Trees To Bring Back The American Chestnut

“Chestnuts Roasting on an Open Fire” is playing on the radio now in the Northern Hemisphere which begs the question, “What happened to the American chestnut?” Would you be surprised to hear there’s a group dedicated to bringing it back from “functional extinction?” [via Inhabitat]

Between logging and the introduction of chestnut blight, the once prevalent American chestnut became increasingly uncommon throughout its traditional range in the Appalachians. While many trees in the southern range were killed by Phytophthora root rot (PRR), the chestnut blight leaves roots intact, so many chestnuts have been surviving by growing back from the roots only to succumb to the blight and be reborn again. Now, scientists are using a combination of techniques to develop blight-resistant trees from this remaining population.

The American Chestnut Foundation recognizes you can’t improve what you can’t measure and uses a combination of “small stem assays (SSAs) performed on potted seedlings, improved phenotype scoring methods for field-grown trees, and the use of genomic prediction models for scoring resistance based on genotype.” This allows them to more rapidly screen varieties for blight resistance to further their efforts. One approach is based on conventional plant breeding techniques and has been crossing blight and PRR-resistant Chinese chestnuts with the American type. PRR resistance has been found to be less genetically complicated, so progress has been faster on resistance to that particular problem. Continue reading “Hacking Trees To Bring Back The American Chestnut”

Open-Source Insulin: Biohackers Aiming For Distributed Production

When you’ve got a diabetic in your life, there are few moments in any day that are free from thoughts about insulin. Insulin is literally the first coherent thought I have every morning, when I check my daughter’s blood glucose level while she’s still asleep, and the last thought as I turn out the lights, making sure she has enough in her insulin pump to get through the night. And in between, with the constant need to calculate dosing, adjust levels, add corrections for an unexpected snack, or just looking in the fridge and counting up the number of backup vials we have on hand, insulin is a frequent if often unwanted intruder on my thoughts.

And now, as my daughter gets older and seeks like any teenager to become more independent, new thoughts about insulin have started to crop up. Insulin is expensive, and while we have excellent insurance, that can always change in a heartbeat. But even if it does, the insulin must flow — she has no choice in the matter. And so I thought it would be instructional to take a look at how insulin is made on a commercial scale, in the context of a growing movement of biohackers who are looking to build a more distributed system of insulin production. Their goal is to make insulin affordable, and with a vested interest, I want to know if they’ve got any chance of making that goal a reality.

Continue reading “Open-Source Insulin: Biohackers Aiming For Distributed Production”

Genetically Modified Mosquitos: Biohacking For Disease Prevention

Many years ago, I took a summer trip to the Maryland shore with some friends. One of my buddies and I got bored with playing football on the beach, so we decided to take a hike on one of the many trails back into the wooded area behind the dunes. At the trailhead we noticed a prominent sign, warning about the presence of “very aggressive mosquitos” and not to enter without first applying ample insect repellent. We scoffed at the warning as only young idiots could and soldiered on, bare-legged and confident that we’d be fine.

About three minutes into our hike, a small group came pelting down the trail in a panic. “It’s true! Turn back!” they shouted as they flew past us. Undeterred, or at least unwilling to appear that way to each other, we pressed on, only to discover a few minutes later that we were making a substantial blood sacrifice to the next generation of mosquitos on Assateague Island. We couldn’t bear more than a few seconds before turning tail and running back to the beach and jumping into the ocean to get rid of the last few dozen bloodsuckers.

I learned a valuable lesson from that experience, as well as developing a deep and abiding hatred of mosquitos. It turns out I’m in good company — pretty much everyone hates mosquitos, which are not just a nuisance but can be downright dangerous to be around. But if tests with genetically engineered mosquitos currently underway in Florida turn out well, we may be able to finally turn the tide against mosquito-borne diseases, simply by killing all the females before they ever reach adulthood.

Continue reading “Genetically Modified Mosquitos: Biohacking For Disease Prevention”

Ctrl-X, Ctrl-V For DNA

Once upon a time, the aspiring nerdling’s gift of choice was the Gilbert chemistry set. Its tiny vials of reagents, rack of test tubes, and instruction book promised endless intellectual stimulation and the possibility of stink bombs on demand. Now a new genetic engineering lab-in-a-box Kickstarter, with all the tools and materials needed to create your own transgenic organisms, may help the young biohacker’s dreams come true.

The Kickstarter has been wildly successful. The initial goal was $1200AUD was met in a day, and currently stands at almost $6200AUD. Despite that success, color me skeptical on this one. Having done way more than my fair share of gene splicing, there seem to be a few critical gaps in this kit. For example, the list of materials for the full kit includes BL21 competent E. coli as the host strain. Those cells are designed to become porous to extracellular DNA when treated with calcium chloride and provided with a heat shock of 42°C. At a minimum I’d think they’d include a thermometer so you can control the heat shock process. Plenty of other steps also need fairly precise incubations, like the digestion and ligation steps needed to get your gene into the host. And exactly what technique you’d be using to harvest DNA from the animal, plant or fungal cells is unclear; the fact that most of the techniques for doing so require special techniques leads me to believe there’s a lot less here than meets the eye.

To be fair, I’ve been off the lab bench for the better part of two decades, and the state of the art has no doubt advanced in that time. There could very well be techniques I’m not familiar with that make the various steps needed to transform a bacterial culture with foreign DNA trivial. It could also be the case that the techniques I used in the lab were optimized for yield and for precise data, while the GlowGene kit provides the materials to get a “good enough” result. I hope so, because a kit like this could really expand the horizons of hackerdom and start getting the biohacking movement going.

[Thanks, Michael!]