Laser Etching PCBs

A while ago, [Marco] mounted a powerful laser diode to a CNC machine in an attempt to etch copper clad board and create a few PCBs. The results weren’t that great, but the technique was promising. In a new experiment, [Marco] purchased a very cheap laser engraver kit from China, and now this technique looks like it might be a winner.

[Marco] sourced his laser engraver from Banggood, and it’s pretty much exactly what you would expect for a CNC machine that costs under $200. The frame is aluminum extrusion, the motors are off-the-shelf steppers, the electronics are just Pololu-like drivers, and the software is somewhere between abysmal and terrible. Nevertheless, this machine can cut wood, leather, fabric, and can remove spray paint with a big blue laser diode.

To create his PCBs, [Marco] is first cleaning a piece of copper clad board, coating it with spray paint, then blasting it with a laser. The preferred software for this is LaserWeb, and the results are pretty good for a cheap machine.

There are a few extra steps to creating the PCB once the board has been coated with paint and blasted with a laser. This process still requires etching in either ferric chloride or some other mess of acid, but the results are good. So good, in fact, that [Marco] is experimenting with copper foil and Kapton to create flexible circuit boards. You can check out the video of these experiments below.

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Electric Longboard with All-New Everything

We love [lolomolo]’s Open Source electric longboard project. Why? Because he completely re-engineered everything while working on the project all through college. He tackled each challenge, be it electronic or mechanical as it came, and ended up making everything himself.

The 48″ x 13″ deck is a rather unique construction utilizing carbon fiber and Baltic birch. In testing the deck, [lolomol] found the deflection was less than an inch with 500 lbs. on the other end. He modified the Caliber II trucks to add four 2250W Turnigy Aerodrive brushless outrunners driving the wheels with the help of belts. The motors are controlled by VESC, an Open Source speed controller. There are a lot of fun details, like the A123 lithium cells equipped with custom battery management system PCBs.

The board sports 5W RGBW headlights that are so bright he can only run them at 10% PWM, plus RGB LED underlighting. All of it is controlled by an onboard Linux box. You can check out [lolomolo]’s GitHub repository for code, schematics, and CAD files. His Instructable for this project also has more design notes and thoughts.

If sweet longboards are your bag, check out the 3D-printed longboard and the long-distance electric longboard we published previously.

A Detailed Guide for 3D Printing Enclosures

We’ve all have projects that are done, but not complete. They work, but they’re just a few PCBs wired together precariously on our desks. But fear not! A true maker’s blog has gifted us with a detailed step-by-step guide on how to make a project enclosure.

Having purchased an MP Select Mini 3D Printer, there was little to do but find something practical to print. What better than an enclosure for a recently finished Time/Date/Temperature display Arduino based device?

The enclosure in this guide, while quite nice, isn’t the main attraction here. The real feature is the incredibly detailed instructions for how to design, model and print an enclosure for any project. For the veterans out there, it seems simple. Sketch something on the back of a napkin and take a nap on your keyboard with OpenSCAD open. When you wake, BAM: perfect 3D model. However, for newcomers, the process can seem daunting. With incredibly specific instructions (an example is “Open up a new workspace by clicking CREATE NEW DESIGN,” notice the accurate capitalization!), it should ease the barrier of the first enclosure, turning the inexperienced into the kind-of-experienced.

If you’ve been printing enclosures since the dawn of time or plastic simply isn’t your style, boy, do we have you covered. Why not check out FR4 (aka PCB) enclosures? Or what about laser cut enclosures from eagle files? Maybe two-piece boxes are more your thing.

Open Source Modular Rocket Avionics Package

Cambridge postgraduate student [Adam Greig] helped design a rocket avionics system consisting of a series of disc-shaped PCBs arranged in a stack. There’s a lot that went into the system and you can get a good look at it all through the flickr album.

Built with the help of Cambridge University Spaceflight, the Martlet is a 3-staging sounding rocket that lifts to 15km/50K feet on Cesaroni Pro98 engines. [Adam]’s control system uses several Arm Cortex M4s on various boards rather than having just one brain controlling everything.

Each disc is a module that plays a specific role in the system. There are a couple of power supply boards sporting twin LTC2975 able to supply custom power to a dozen different circuits. The power system has a master control board also sporting an M4. There’s an IMU board with the guidance system — accelerometer, magnetometer, gyroscope, and barometer, all monitored by an algorithm that computes the rocket’s position and attitude in-flight. There’s a radio board with a GPS receiver and an ISM band radio transceiver for telemetry, as well as a datalogger with 10 thermocouple measurement channels. Engines are controlled by the pyro board which controls firing currents on four different channels. The vertical spacers also serve to transmit power and data to neighboring boards.

If you’re interested in learning more, check out the project’s code and schematics on [Adam]’s GitHub repository.

[Adam] is no stranger to these pages, with his Nerf Vulcan turret published a few years back, as well as his balloon tracking rig published more recently. Photos are CC-SA and can be found in [Adam]’s Flickr feed.

Horizontal Magnetic Levitation Experiments

Levitating chairs from the Jetsons still have a few years of becoming a commercial product though they are fun to think about. One such curious inventor, [Conor Patrick], took a deep dive into the world of maglev and came up with a plan to create a clock with levitating hands. He shares the first part of his journey to horizontal levitational control.

[Conor Patrick] bought an off-the-shelf levitation product that was capable of horizontal levitation. Upon dissecting it he found a large magnet, four electromagnet coils, and a hall effect sensor. These parts collectively form a closed-loop control to hold an object at a specific distance. He soon discovered that in fact, there were just two coils energized by H-bridges. His first attempt at replicating the circuit, he employed a breadboard which worked fine for a single axis model. Unfortunately, it did not work as expected with multiple coils.

After a few iteration and experiments with the PID control loop, he was able to remove unwanted sensor feedback as well as overshoot in control current. He finally moved to a Teensy with a digital PD loop. The system works, but only marginally. [Conor Patrick] is seeking help from the control loop experts out there and that is the essence of the OSHW world. The best part of this project is that it is a journey that involves solving one problem at a time. We hope to see some unique results in the future.

We have covered Acoustic Levitation in the past and the Levitating Clock on a similar beat. We’re certain a more refined approach is on the horizon since many of us are now looking at making one to experiment with on our workbench.

Hackaday Prize Entry: MappyDot, a Micro Smart LiDAR Sensor

[Blecky]’s entry to the Hackaday Prize is MappyDot, a tiny board less than a square inch in size that holds a VL53L0X time-of-flight distance sensor and can measure distances of up to 2 meters.

MappyDot is more than just a breakout board; the ATMega328PB microcontroller on each PCB provides filtering, an easy to use  I2C interface, and automatically handles up to 112 boards connected in a bus. The idea is that one or a few MappyDots can be used by themselves, but managing a large number is just as easy. By dotting a device with multiple MappyDots pointing in different directions, a device could combine the readings to gain a LiDAR-like understanding of its physical environment. Its big numbers of MappyDots [Blecky] is going for, too: he just received a few panels of bare PCBs that he’ll soon be laboriously populating. The good news is, there aren’t that many components on each board.

It’s great to see open sourced projects and tools in which it is clear some thought has gone into making them flexible and easy to use. This means they are easier to incorporate into other work and helps make them a great contestant for the Hackaday Prize.

Living Logic: Biological Circuits for the Electrically Minded

Did you know you can build fundamental circuits using biological methods? These aren’t your average circuits, but they work just like common electrical components. We talk alot about normal silicon and copper circuits ‘roud here, but it’s time to get our hands wet and see what we can do with the power of life!

In 1703, Gottfried Wilhelm Leibniz published his Explication de l’Arithmétique Binaire (translated). Inspired by the I Ching, an ancient Chinese classic, Leibniz established that the principles of arithmetic and logic could be combined and represented by just 1s and 0s. Two hundred years later in 1907, Lee De Forest’s “Audion” is used as an AND gate. Forty years later in 1947, Brattain and H. R. Moore demonstrate their “PNP point-contact germanium transistor” in Bell Labs (often given as the birth date of the transistor). Six years later in 1953, the world’s first transistor computer was created by the University of Manchester. Today, 13,086,801,423,016,741,282,5001 transistors have built a world of progressing connectivity, automation and analysis.

While we will never know how Fu Hsi, Leibniz, Forest or Moore felt as they lay the foundation of the digital world we know today, we’re not completely out of luck: we’re in the midst’s of our own growing revolution, but this one’s centered around biotechnology. In 1961, Jacob and Monod discovered the lac system: a biological analog to the PNP transistor presented in Bell Labs fourteen years earlier. In 2000, Gardner, Cantor, and Collins created a genetic toggle switch controlled by heat and a synthetic fluid bio-analog2. Today, AND, OR, NOR, NAND, and XOR gates (among others) have been successfully demonstrated in academic labs around the world.

But wait a moment. Revolution you say? Electrical transistors went from invention to computers in 6 years, and biological transistors went from invention to toggle button in 40? I’m going to get to the challenges facing biological circuits in time, but suffice it to say that working with living things that want to be fed and (seem to) like to die comes with its own set of challenges that aren’t relevant when working with inanimate and uncaring transistors. But, in the spirit of hacking, let’s dive right in.

My First BioCircuit™

Following the Composition Model (even though the Network Layer Model might be preferred), I’m going to go through the construction of a simple oscillator through electronic and biological methods. The goal is by the end you know a bit more about biological circuits, what they’re made of, what they can do and why they’re hard to make.

DNA/Physical Makeup

In electronics, we make our parts (for the most part) out of copper, silicon, and epoxy. In biology, the base parts are strands of DNA3. For this reason, if you need a quick refresher on DNA, take it. But, all you must know for this article is that DNA encodes genetic information.

Part List: Protein Expression Mediated Logic

For our oscillator, we’re going to need three inverting gates. In the electronics world, that’s pretty easy (like $0.06 easy). You can make electronic logic gates out of (almost) anything and there are countless resources detailing every… intricate… detail that any sane person would want to know.

The biological world has its fair share of logic gates, some of which we’ve already covered. We’re going to focus on the most common type: protein expression mediated logic. Through this method, protein concentrations are treated as signal and are used just like electrical signals are. Below is a genetic NOT gate, signal A (a specific protein for the Promoter or “input”) inhibits the output of signal B (the protein that the “Gene of Interest” codes for). This works just like a traditional NOT gate, if there is not signal A, then produce signal B. Well, it works almost the same way as a traditional NOT gate. This is where we find one of the great challenges that synthetic biology faces: orthogonality.

In electrical logic, we don’t usually need to worry about orthogonality, as we can connect the output of a NOT gate to what we want (and only what we want) with wires and/or traces. In biology, we don’t usually have this luxury as we’re working in prokaryotes (cells without nuclei) which can be effectively thought of as bags of chemicals4 So, instead of having direct connections from one gate to another, we only have one signal “net” as part of our network.

Computers have this figured out with identifiers like MAC addresses. For example, if Bob wants to send a message to Sue, he might broadcast something like “FOR: SUE, MESSAGE: HI”. This works out great for computers: they craft their own receivers and transmitters. In code and hardware, it can be trivial (that’s part of the reason why we have so many electronics communication standards ) but, in biology protein engineering is hard, and it takes time, ingenuity and labor.

Imagine that you could only use one type of transistor in each project. As an example, consider that if you used two 2N3904s, you turn both on or off, but never one off and one on. Now, pretend that for each transistor, you had to corner a computer in the wild, disassemble it and hand remove all of its components in the hopes of finding a new type of transistor. Surely, that would make creating a computer with 30 billion transistors, in one word, difficult.

As such, practically all of the promoters5 (input sites, coded in DNA) that are used today were originally discovered in nature and have been adapted for synthetic use. And, we’ve been pretty successful at this, with enough usable promoters that we need a catalog to sort them all out6.

Device Built with NOT Gates

Now, ever forward. Let’s try to build a device with our NOT gates. And, as we’re really just here to learn concepts, I’m going to show you how to create a ring-oscillator. If you’re a bit rusty on your terminology, or you’ve been too busy to bother with a 100 level circuits class, no worries. A ring-oscillator is really simple, which is why it was one of the first bio-oscillators made. Ring-oscillators are made by placing an odd number of inverters (usually greater than 1) in a cycle (ring). See below for a very cool gif™ that hopefully should clear up any doubts about how it works. But, if things aren’t crystal clear, Enrique from MITx has your back.

Now for the bio part. We already have our NOT gates, so surely we can string three identical NOT gates together and end up with an oscillator, right? Well, as we covered earlier, we’ll need to use three different gates, so things look like three NOT gates in parallel, rather than in series. But, even with three different NOT gates, we might have another problem: death.

After all, all our logic is taking place in a cell using up resources and producing a “useless” protein. And, by the very nature of cells, there’s a limit to the number of resources a cell has. We can try to improve our cell’s effective throughput, but at some point, we need to realize that the cell, as greedy as it is, must take care of the basic requirements of life. However, fear not. Our three gate oscillator should be sustainable7. But, if we were to try seven or even nine gate oscillators we might need to start worrying.

Anyway, enough dilly dallying. We’ve put our DNA sequences together, introduced them into a cell and now we wait for them to grow8. We’ve used GFP (a Green Fluorescent Protein) as one of our “signals” so we can visualize our signal (just like one would use an LED to view a digital signal).

And… nothing. At first. After about 160 minutes our cells start to glow. Just like electronic ring-oscillators, our frequency depends on the delay time of each NOT gate. In electrical contexts, this gives us frequencies commonly in/above the kHz range. In biology, we’re working at a blazing fast 1×10[-4] Hz with a period of ~160 minutes. Again, another stark difference between biological and electrical circuits.

In Practice / Why You Should Care

Okay, I know how it seems. Biological Logic is underdeveloped, slow and constrained by life. But, there is a harmony! What biology lacks, it makes up for in immeasurable ways. After all, we’re made of cells, not silicon.

From regulating transgene expression for regenerative medicine to a tunable dual-promoter integrator for targeting of cancer cells, cellular logic has the opportunity to change the way we think about how medicine should work, what we think life is and whether or not plant’s should glow. You should care because it’s cool it’s new and it has the potential to help lots of people. We’re hackers. We’ve always been at the forefront of technology, and just because this one’s squishy things don’t need to change. So, let’s get hacking!

I really enjoy writing about synthetic biology, as you might have guessed, so let me know if there are any topics that y’all would like to hear about. Would a few “Getting Started” articles on, well, getting started in synthetic biology with actionable procedures be a good place to start?

Notes

[1] Calculated using the figures from the Forbes Article “How Many Transistors Have Ever Shipped?”. While I have little confidence in this article, I am willing to believe that the figure presented is accurate to at least a few orders of magnitude. Regardless, the exact number is less important than the overall impression that many transistors surround us today.

[2] Specifically, the E. Coli was controlled by Isopropyl β-D-1-thiogalactopyranoside (IPTG) which is a molecular mimic of allolactose, a lactose metabolite. IPTG is used because, unlike allolactose, IPTG is not hydrolysable by β-galactosidase, so its concentration remains constant as it’s not broken down as readily.

[3] Okay, yes: DNA is made of nucleotides but copper is made of atoms and we have to stop somewhere. In our context today, it makes sense to stop at DNA, but in other contexts, such as DNA synthesis, DNA clearly isn’t the base unit.

[4] To say that cells are just “bags of chemicals” is a gross oversimplification analogous to calling our bodies just “skin with stuff inside”. Technically true, but not very descriptive. What I’m trying to get across here is that cells are (1) bound by a membrane, that they are (2) one system of transmitters and receivers and that (3) they have a limited rate at which they can absorb/deport things. This metaphor makes it easy to visualize these attributes, so I have chosen it in the spirit of Upaya (where something may not be ultimately “true” in the highest sense, but it may still be an expedient practice). For a more accurate representation of cells, check out this award winning book: Cells, Gels and the Engines of Life.

[5] Throughout this article, I use Promoter as a broad phrase, to refer to Repressors and Promoters for two reasons: simplicity and to avoid the constant use of promoters/repressors throughout the article, promoter is already a long enough word.

[6] Granted most, if not all of these promoters are only characterized for E. Coli. But, with E. Coli being the workhorse of synthetic biology, this usually isn’t an issue.

[7] By sustainable, I mean that the cell shouldn’t die from expressing the proteins that we need for our circuit. I, however, did not mean that the output would be sustainable over extended periods of time. As time progresses, our system will break down we’ll eventually get weak expression of all 3 proteins in our system. Oh well, that’s life in the city.

[8] I dislike the fact that I fast forwarded past this process. The process of getting DNA ready (not to mention preparing/designing the DNA) and coercing the cells to express your DNA is a whole ‘nother bucket of worms. Could easily be another article (or set of articles). (Wink wink)