We’ve all had to shake jars of nail polish, model paint, or cell cultures. Mixing paint is easy – but bacteria and cells need to be agitated for hours. Happily, laboratory tube tumblers automate this for us. The swishing action is handled with rotation. The vials are mounted at angles around a wheel. The angular offset means the tubes are inclined as they rise, and declined as they fall. This causes the liquid in the tube to slosh from one side to the other as the wheel rotates. [Sebastian S. Cocioba] aka [ATinyGreenCell] released his plans through Tinkercad and GitHub, and with a name like Sir Tumbalot, we know he must be cultured indeed.
Grab your monocles. Version 2 features a driven wheel lined with magnets to attach tube adapters, and he’s modeled 50mL and twin 15mL tube holders. The attachment points look like a simple beveled rectangle with a magnet pocket, so if you’re feeling vigorous for vials, you can whip up custom sockets and tumble any darn thing. A Trinamic StealthChop chip on a custom PCB controls the pancake stepper, and the whole shebang should cost less than $50USD. We’re wondering what other purposes this modular design could have, like the smallest rock tumbler or resin print rinser.
Making lab equipment is phenomenal for saving money for things that just spin up to a biotech lab.
Continue reading “Tube Tumbler Provides The Perfect Culture”
We like to think our readers are on the cutting edge. With the advent of CRISPR kits at home and DIY bio blooming in workshops across the world, we wanted to share a video which may be ahead of its time. [The Thought Emporium] has just shown us a way to store eukaryotic cells at room temperature. His technique is based on a paper published in Nature which he links to from the YouTube page, but you can see his video after the break.
Eukaryotic cells, the kind we are made of, have been transported at low temperatures with techniques like active refrigeration, liquid nitrogen, and dry ice but those come with a host of problems like cost, convenience, and portability. Storing the cells with cryogel has been shown to reliably keep the cells stable for up to a week at a time and [The Thought Emporium] made some in his homemade freeze-dryer which he’s shown us before. The result looks like a potato chip, but is probably less nutrious than astronaut ice cream.
If cell transport doesn’t tickle your fancy, cryogel is fascinating by itself as a durable, lightweight insulator similar to Aerogel. You can make Aerogel at home too.
Continue reading “DIY Cryogel Sustains Live Cells”