When you think of high-throughput ptychographic cytometry (wait, you do think about high throughput ptychographic cytometry, right?) does it bring to mind something you can hack together from an old Blu-ray player, an Arduino, and, er, some blood? Apparently so for [Shaowei Jiang] and some of his buddies in this ACS Sensors Article.
For those of you who haven’t had a paper accepted by the American Chemical Society, we should probably clarify things a bit. Ptychography is a computational method of microscopic imaging, and cytometry has to do with measuring the characteristics of cells. Obviously.
Anyway, if you shoot a laser through a sample, it diffracts. If you then move the sample slightly, the diffraction pattern shifts. If you capture the diffraction pattern in each position with a CCD sensor, you can reconstruct the shape of the sample using breathtaking amounts of math.
One hitch – the CCD sensor needs a bunch of tiny lenses, and by tiny we mean six to eight microns. Red blood cells are just that size, and they’re lens shaped. So the researcher puts a drop of their own blood on the surface of the CCD and covers it with a bit of polyvinyl film, leaving a bit of CCD bloodless for reference. There’s an absolutely wild video of it in action here.
A robot that performs surgery is a serious thing. One bug in the control system could end with disaster. Unless of course, you’re [Michael Reeves], in which case disaster is all part of the fun. (Video, embedded below.)
Taking inspiration from The da Vinci Surgical System, [Michael] set out to build a system that was faster, while still maintaining precision. He created a belt drive gantry system, not unlike many 3D printers, laser cutters, or woodworking CNC machines. Machines like this often use stepper motors. [Michael] decided to go with [Oskar Weigl’s] ODrive and brushless motors instead. The ODrive is on open source controller which turns off the shelf brushless motors — such as those found in R/C planes or hoverboards, into precision industrial servos. Sound familiar? ODrive was an entrant in the 2016 Hackaday Prize. [Michael] was even able to do away the ubiquitous limit switch by monitoring current draw with the ODrive.
It all adds up to a serious build. But this is [Michael “laser eye” Reeves] after all. The video is meant to be entertaining, with a hidden payload of education and inspiration. The fun starts when he arms the robot with a giant kitchen knife and performs “surgery” on a pineapple. If you want to know what happens when mannequins and fake blood enter the picture, then watch the video after the break.
Being a cop’s kid leaves you with a lot of vivid memories. My dad was a Connecticut State Trooper for over twenty years, and because of the small size of the state, he was essentially on duty at all times. His cruiser was very much the family vehicle, and like all police vehicles, it was loaded with the tools of the trade. Chief among them was the VHF two-way radio, which I’d listen to during long car rides, hearing troopers dispatched to this accident or calling in that traffic stop.
One very common call was the blood relay — Greenwich Hospital might have had an urgent need for Type B+ blood, but the nearest supply was perhaps at Yale-New Haven Hospital. The State Police would be called, a trooper would pick up the blood in a cooler, drive like hell down I-95, and hand deliver the blood to waiting OR personnel. On a good day, a sufficiently motivated and skilled trooper could cover that 45-mile stretch in about half an hour. On a bad day, the trooper might end up in an accident and in need of blood himself.
Building a battery out of common household products is actually pretty simple. All that is required is two dissimilar metals and some sort of electrolyte to facility the transfer of charge. A popular grade school science experiment demonstrates this fairly well by using copper and zinc plates set inside a potato or a lemon. Almost anything can be used as the charge transfer medium, as [dmitry] demonstrates by creating a rather macabre battery using his own blood.
The battery was part of an art and science exhibition but it probably wouldn’t be sustainable on a large scale, as it took [dmitry] around 18 months to bank enough blood to make a useful battery. Blood contains a lot of electrolytes that make it perfect for this application though, and with the addition of the copper anode and aluminum cathode [dmitry] can power a small speaker which plays a sound-generating algorithm that frankly adds a very surreal element to the art installation.
While we can’t recommend that you try to build one of these batteries on your own without proper medical supervision, the video of the art piece is worth checking out. We’ve seen a few other hacks that involve blood, but usually they are attempting to use it for its intended purpose rather than as an alternative energy source.
Simple blood tests can lead a doctor toward a diagnosis of blood cancers, like leukemia, lymphoma and myeloma, but to really see what’s going on, he or she needs to go to the source of the problem: the bone marrow. Examining maturing blood cells from the marrow with a microscope is an important step in staging the disease and developing a plan for treatment, but it’s a tedious and error-prone process that requires a doctor to classify and tally a dozen or so different cells based on their size, shape and features. Automated systems like flow cytometry and image analysis software can help, but in an austere environment, a doctor might not have access to these. Luckily, there’s now an on-line app to assist with bone marrow cytometry.
Thanks to [Eduardo Zola], a doctor can concentrate on classifying cells without looking up from the microscope, and without dictating to an assistant. Keys are assigned to the different cell morphologies, and a running total of each cell type is kept. With practice, the doctor should be able to master the keying for the various cells; we suspect the generation of physicians that grew up with the WASD keying common in PC-based gaming might have a significant advantage over the older docs when it comes to learning such an app.
[Eduardo]’s app seems like a simple way to improve on an important medical procedure, and an enabling technology where access to modern instrumentation is limited. To that end, one area for improvement might be a standalone app that can run on a laptop without internet access, or perhaps even a version that runs on a smart phone. But even as it is, it’s a great entry for the 2015 Hackaday Prize.
This pulse oximeter turned out very nicely. It is based around a Freescale microcontroller and detects pulse as well as oxygen saturation in your blood. The sensor is made of two wood pieces and allows two wavelengths of light to be shined through your finger. A sensor picks up the light on the other side of your stubby digit and the readings are compared to calculate saturation. Check out the finished project after the break.
[Mike] is building his own Pulse Oximeter which uses light to measure the oxygen saturation in blood. One collateral benefit of this measurement is that pulse rate can be calculated from the same data. The parts used for the detector include a red LED, infrared LED, and a TSL230R light intensity measuring chip. As explained in the video above, each LED is shined through the tip of your finger and onto the light sensor. The IR LED is used as a baseline and compared to the red LED, which has some of its intensity absorbed by the red blood in your finger. This is a pretty approachable biometric concept so you may want to start here before moving on to more involved biometric interfaces.