Digital cameras have been around for forty years or so, and the first ones were built around CCDs. These were two-dimensional CCDs, and if you’ve ever looked inside a copier, scanner, or one of those weird handheld scanners from the 90s, you’ll find something entirely unlike what you’d see in a digital camera. Linear CCDs are exactly what they sound like — a single line of pixels. It’s great if you’re into spectroscopy, but these linear CCDs also have the advantage of having some crazy resolutions. A four-inch wide linear CCD will have thousands of pixels, and if you could somehow drag a linear CCD across an image, you would have a fantastic camera.
Many have tried, few have succeeded, and [heye.everts]’ linear CCD camera is the best attempt at making a linear CCD camera yet. It took a fuzzy picture of a tree, which is good enough for a proof of concept.
The linear CCD used in this project works something like an analog shift register. With a differential clock, you simply push values out of the CCD and feed them into an ADC. The driver board for this CCD uses a lot of current and the timings are a bit tricky but it does work with a Teensy 3.6. But that’s only one line of an image, you need to move that CCD too. For that, this project uses something resembling a homebrew CD drive. There’s a tiny stepper motor and a leadscrew dragging the CCD across the image plane. All of this is attached to the back of a Mamiya RZ67 camera body.
Does it work? Yes. Surprisingly yes. After a lot of work, an image of a tree was captured. This is an RGB CCD, and at the moment it’s only using one color channel, but it does work. It’s a proof of concept rendered in a 2000 x 3000 grayscale bitmap. The eventual goal is to build a 37.5 Megapixel medium format camera around this CCD, and the progress is looking great.
Ever since a Dutch businessman peered into the microscopic world through his brass and glass contraption in the 1600s, microscopy has had a long, rich history of DIY innovation. This DIY fluorescence microscope is another step along that DIY path that might just open up a powerful imaging technique to amateur scientists and biohackers.
In fluorescence microscopy, cells are treated with various fluorescent dyes that can be excited with light at one wavelength and emit light at another. But as [Jonathan Bumstead] points out, fluorescence microscopes are generally priced out of the range of biohackers. His homebrew scope levels the playing field a bit. The trick is to use 3D-printed parts to kit out commonly available digital cameras – a USB microscope, a DSLR, or even a smartphone camera. Excitation is provided by a ring of Nexopixel LEDs, while a movable rack holds a filter that blocks the excitation wavelength but allows the emission wavelength to pass through to the camera. He demonstrates the technique by staining some threads with fluorescent ink from a highlighter marker and placing them on a sheet of tissue paper; in conventional bright-field mode, the threads all but disappear into the background, but jump right out under fluorescence.
Ith’s true that the optics are not exactly lab quality, and the microscope is currently only set up to do reflectance imaging as opposed to the more typical transmissive mode where the light passes through the sample. That’s an easy fix, though, and reflectance mode is still useful. We’ve seen fluorescence microscopy get quite complex before, but this simple scope might be enough to get a biohacker started.
Continue reading “See Cells In A New Light With A DIY Fluorescence Microscope”
Improvements in methodology have dramatically dropped the cost of DNA sequencing in the last decade. In 2007, it cost around $10 million dollars to sequence a single genome. Today, there are services which will do it for as little as $1,000. That’s not to bad if you just want to examine your own DNA, but prohibitively expensive if you’re looking to experiment with DNA in the home lab. You can buy your own desktop sequencer and cut out the middleman, but they cost in the neighborhood of $50,000. A bit outside of the experimenter’s budget unless you’re Tony Stark.
But thanks to the incredible work of [Alexander Sokolov], the intrepid hacker may one day be able to put a DNA sequencer in their lab for the cost of a decent oscilloscope. The breakthrough came as the result of those two classic hacker pastimes: reverse engineering and dumpster diving. He realized that the heavy lifting in a desktop genome sequencer was being done in a sensor matrix that the manufacturer considers disposable. After finding a source of trashed sensors to experiment with, he was able to figure out not only how to read them, but revitalize them so he could introduce a new sample.
To start with, [Alexander] had to figure out how these “disposable” sensors worked. He knew they were similar in principle to a digital camera’s CCD sensor; but rather than having cells which respond to light, they read changes in pH level. The chip contains 10 million of these pH cells, and each one needs to be read individually hundreds of times to capture the entire DNA sequence.
Enlisting the help of some friends who had experience reverse engineering silicon, and armed with an X-Ray machine and suitable optical microscope, he eventually figured out how the sensor matrix worked electrically. He then designed a board that reads the sensor and dumps the “picture” of the DNA sample to his computer over serial.
Once he could reliably read the sensor, the next phase of the project was finding a way to wash the old sample out so it could be reloaded. [Alexander] tried different methods, and after several wash and read cycles, he nailed down the process of rejuvenating the sensor so its performance essentially matches that of a new one. He’s currently working on the next generation of his reader hardware, and we’re very interested to see where the project goes.
This isn’t the first piece of DIY DNA hardware we’ve seen here at Hackaday, and it certainly won’t be the last. Like it or not, hackers are officially fiddling with genomes.
Arduino 101 is getting an LED to flash. From there you have a world of options for control, from MOSFETs to relays, solenoids and motors, all kinds of outputs. Here, we’re going to take a quick look at some inputs. While working on a recent project, I realized the variety of options in sensing something as simple as whether a light is on or off. This is a fundamental task for any system that reacts to the world; maybe a sensor that detects when the washer has finished and sends a text message, or an automated chicken coop that opens and closes with the sun, or a beam break that notifies when a sister has entered your sacred space. These are some of the tools you might use to sense light around you.
Continue reading “Is It On Yet? Sensing The World Around Us, Starting With Light”
Ever wanted to build a touch table or other touch-input project, but got stuck figuring out the ‘touch’ part? [Jean Perardel] has your back with his multi-touch frame over on IO that makes any surface touch-reactive. In [Jean]’s case, that surface is ultimately a TV inside of a table.
Of course, it’s a bit of a misnomer to say the surface itself becomes touch-reactive. What’s really happening here is that [Jean] is using light triangulation to detect shadows and determine the coordinates of the shadow-casting object. Many barcode scanners and consumer-level document scanners use a contact image sensor (CIS) to detect objects in the path of IR LEDs. These are a low-power, lower-resolution alternative to the CCDs found in high-grade scanners.
As [Jean] explains in the video below, an object placed in the path of a single IR LED facing a sensor array of either type will block the light from reaching the sensors. Keep adding LEDs and their emission angles will begin to overlap, increasing the detection precision. [Jean] reverse engineered a couple of different types of scanners until he found a suitable one. He ended up with CIS that has 2700 light sensors lined up in the space of 20cm (7.87″).
[Jean] designed a 3D-printable frame to hold 96 IR LEDs in stacks of three. A Teensy turns on the LEDs, detects the touch event, calculates the position, and sends those coordinates to a Pi to be displayed on the screen. He eventually went wireless and then built a nice looking touch table to house a 32″ TV.
This is not the only way to build a multi-touch table, nor is it the simplest. Here’s one that uses finger presses to scatter light and an industrial strength projection-based table that was open-sourced a few years ago.
Continue reading “DIY Multi-Touch All The Surfaces”
It’s a problem as old as photography: your camera is only as good as your lens. As cameras shrink, so do lenses, and so do the options for upgrading to a better lens. And forget about switching to a different focal length or aperture — it’s often just not an option. Unless you make it an option by adding a CS lens mount to a high-end webcam.
We’ll stipulate that at 4k resolution and packed with all sorts of goodies, the Logitech Brio Pro is a heck of a nice camera. And the lens isn’t bad either, as you’d hope for a camera with almost 9 megapixels at its disposal. But with an optical field of view optimized for video conferencing, it’s hard to use this premium camera for much else. [Saulius] fixed that by taking the camera apart and adding a new case with a built-in C- and CS-mount, resulting in literally thousands of lens choices. [Saulius]’ post has valuable teardown information, which includes exposing the CCD sensor completely. The new case is sold as a kit, but it looks like a 3D-printed case would be pretty easy to whip up.
[Salius] sure seems to love those optical hacks, whether they be a budget microscope camera, high-resolution LIDAR, or capturing license plates at great distances.
While most people who make the trek to the path of totality for the Great American Eclipse next week will fix their gazes skyward as the heavenly spectacle unfolds, we suspect many will attempt to post a duck-face selfie with the eclipsed sun in the background. But at least one man will be feverishly tending to an experiment.
On a lonely hilltop in Wyoming, Dr. Don Bruns will be attempting to replicate a famous experiment. If he succeeds, not only will he have pulled off something that’s only been done twice before, he’ll provide yet more evidence that Einstein was right.
Continue reading “Eclipse 2017: Was Einstein Right?”