Quick And Dirty Microscope Motion Control For Focus Stacking

If you’ve spent much time looking through a microscope, you know that their narrow depth of field can be a bit challenging to deal with. Most microscopes are designed to only have a very thin slice of the specimen in focus, so looking at anything above or below that plane requires a focus adjustment. It’s tedious and fussy, and that makes it a perfect target for automation.

The goal behind [ItMightBeWorse]’s microscope mods is “focus stacking,” a technique where multiple images of the same sample taken at different focal planes can be stitched together so that everything appears to be in focus. Rather than twist knobs and take pictures manually, he built a simpler Arduino-based rig to do the job for him. Focus control is through a small stepper motor connected to the fine focus knob of the scope, while the DSLR camera shutter is triggered through a simple relay board. There’s also lighting control, with an RGB LED ring light that can change both the light level on the sample as well as the tint.

The code is very simple, and the setup is quite temporary looking, but the results are pretty impressive. We could do without the extreme closeup of that tick — nasty little arachnids — but the ant at the end of the video below has some interesting details. [ItMightBeWorse] doesn’t mention how the actual stacking is being done, but this CNC-based focus stacking project mentions a few utilities that take help with the post-processing.

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Bode Plot Un-Lecture

[Rolinychupetin] insists that his recent video is not a lecture but actually a “recitation” about Bode plots. That may be, but it is still worth a watch if you want to learn more about the topic. You can see the video below.

If you haven’t run into Bode plots before, they are simple plots of magnitude or phase vs. frequency, usually plotted on a log scale. Named after Bell Lab’s [Hendrik Wade Bode], they are useful for understanding filters or anything with a frequency response.

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Tidy Breadboard Uses Banana Bread

Self-described passionate maker in the electronics and 3D printing world, [Jakob], aka [testudor], was getting frustrated trying to connect banana plugs to solderless breadboards. Project Banana Bread was born — small banana jack adaptors and a companion tray with pockets to hold up to six modules.

The base in the photo is made from 5083 aluminum, machined on a homemade CNC router. But design files for a yet-to-be-tested 3D printer version are available as well. As can happen, he strayed from the original goal of solving the banana jack issue, and also cranked out a USB-serial port and a blank template module for any custom interfaces folks may want to implement.

If it is only power connections you are interested in, we covered the Open Power project back in 2019. And also don’t forget the mother of all breadboards, this 1960s behemoth we wrote about last year. What kinds of breadboard interface modules do you find most useful? Let us know in the comments below.

 

DIY Mini Fridge Is Pure Brilliance In Foam

There’s nothing more pleasing on a hot day than an ice-cold beverage. While the vast majority of us have a fridge in the kitchen, sometimes it’s desirable to have a further fridge in the lab, games room, or workshop. To that end, you may find value in this ultra-cheap, low-cost DIY fridge build from [Handy_Bear].

Like many tiny fridge builds, this design eschews complex gas-cycle refrigeration techniques for simple Peltier modules. These are devices that have one cold side and one hot side, because they move heat when electricity is applied. This build uses a Peltier module fitted with a fan to better shift away heat from the hot side, improving the module’s cooling ability.

The “fridge” itself is assembled out of thick XPS insulation foam. A hot wire cutter was used to cut several slabs which were then assembled using hot glue. The Peltier module is installed on the back, at the top of the fridge. Thus, air which is cooled in this area will then travel down through the rest of the fridge’s cavity. [Handy_Bear] also goes over how to produce a working hinge and a gasket for the door, which helps with ease-of-use and efficiency. As a nice touch, a set of 12V LED lights are also installed inside, which light when the door is open. Just like the real thing!

The final build is noisy, slow to cool down, and it uses 60 watts of power to cool down just two regulation-sized sodas. Notably, you could fit two standard NATO smoke grenades in the same space, as they’re almost-identically sized (ask us how we know). However, smoke grenades don’t usually need to be refrigerated.

None of that means it isn’t fun though! Plus, [Handy_Bear] notes that adding a second Peltier would greatly aid the fridge’s ability to quickly chill your grenades sodas. You might even like to explore the use of special fan designs to make the fridge even quieter! Video after the break.

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Decoding 433 MHz Signals With Arduino & Raspberry Pi

433 MHz radio signals are all around us. They’re used for things like smart power plugs, garage door openers, and home weather stations. Decoding these signals can allow you to interface and work with these devices on your own terms. To help in those efforts, [Joonas Pihlajamaa] has written a three-part tutorial on decoding these signals.

A soundcard makes for a very cheap oscilloscope.

The focus of the tutorials is decoding the signals of a Nexa radio-controlled smart plug. [Joonas] first explores using an Arduino to do the job, paired with a RFM210LCF-433D radio receiver module. This setup dumps out data to a computer over serial for decoding. [Joonas] then tried an alternative strategy, using a soundcard as a “poor man’s oscilloscope” to do the same job, using the same radio module and using Audacity for signal analysis. Finally, [Joonas] brought out the big guns, hooking up a Picoscope digital oscilloscope to a Raspberry Pi 4 for a more deluxe attempt at decoding the signals.

The tutorial goes to show that higher-end tools can make such a job much easier. However, the cheaper techniques are a great way of showing what can be done with the bare minimum in tools. We’re hoping for an exciting fourth part to [Joonas’s] work, where he instructs us on how to decode 433 MHz signals by drinking huge amounts of caffeine and staring at a very fast blinking LED. If you’ve got your own nifty signal analysis (or SIGINT!) hacks, be a good sport and drop them into the tipsline!

 

Decoding Compact Disc Audio From Scratch

In the rare case we listen to an audio CD these days, we typically rely on off-the-shelf hardware to decode the 1s and 0s into the dulcet tones of Weird Al Yankovic for our listening pleasure. [Lukas], however, was recently inspired to try decoding the pits and lands of a CD into audio for himself.

A fair bit goes into decoding Red Book digital audio.

[Lukas] did the smart thing, and headed straight to the official Red Book Audio CD standard documents freely available on archive.org. That’s a heck of a lot cheaper than the €345 some publishers want to charge. Not wanting to use a microscope to read the individual pits and lands of the disc, [Lukas] used a DVD player. The electrical signals from the optical pickup were captured with an oscilloscope. 4 megasamples of the output were taken at a rate of 20 megasamples per second. This data was then ported over to a PC for further analysis in Python.

[Lukas] steps us through the methodology of turning this raw data of pits and lands into real audio. It’s a lot of work, and there are some confusing missteps thanks to the DVD player’s quirks. However, [Lukas] gets there in the end and shows that he truly understands how Red Book audio really works.

It’s always interesting to see older media explored at the bare level with logic analyzers and oscilloscopes. If you’ve been doing similar investigative work, don’t hesitate to drop us a line! 

Equipping Rats With Backpacks To Find Victims Under Rubble

When it comes to demining or finding victims after a disaster, dogs are well-known to aid humans by sniffing out threats and trapped humans with ease. Less well-known, but no less impressive are rats, with the African giant pouched rat being the star of the show. Recently a student at the Dutch Technical University of Eindhoven (TU/e) has demonstrated how these rats can sniff out buried victims, aided by a high-tech backpack that gives them a communication link back to their human handler.

All of this is done in association with the Belgian-registered and Tanzania-based NGO APOPO, whose achievements include training gold medal winner Magawa the rat, who helped find 71 landmines and dozens more types of UXO over a 5-year career. These landmine-hunting rats are known as HeroRATs and have been helping demine nations since the 1990s. They may be joined by RescueRats in the near future.

Each RescueRat is equipped with a backpack that contains a camera and battery, as well as GPS and altimeter. Each backpack includes a button that the rat is trained to press when they have found a victim — essentially dropping a pin on their human rescuer’s maps.

Figuring out the location of the victim inside the rubble pile is the real challenge. This is where a (LoRa) radio beacon in the backpack is triangulated using receivers placed around the area, allowing the rescuers to determine with reasonable accuracy where to focus their efforts.

(Thanks to [Roel] for the tip!)

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