How Peptides Are Made

What does body building, anti-aging cream and Bleomycin (a cancer drug) have in common? Peptides of course! Peptides are large molecules that are vital to life. If you were to take a protein and break it into smaller pieces, each piece would be called a peptide. Just like proteins, peptides are made of amino acids linked together in a chain-like structure. Whenever you ingest a protein, your body breaks it down to its individual amino acids. It then puts those amino acids back together in a different order to make whatever peptide or protein your body needs. Insulin, for instance, is a peptide that is 51 amino acids long. Your body synthesizes insulin from the amino acids it gets from the proteins you eat.

Peptides and small proteins can be synthesized in a lab as well. Peptide synthesis is a huge market in the pharmaceutical and skin care industry. They’re also used, somewhat shadily, as a steroid substitute by serious athletes and body builders. In this article, we’re going to go over the basic steps of how to join amino acids together to make a peptide. The chemistry of peptide synthesis is complex and well beyond the scope of this article. But the basic steps of making a peptide are not as difficult as you might think. Join me after the break to gain a basic understanding of how peptides are synthesized in labs across the world, and to establish a good footing should you ever wish to delve deeper and make peptides on your own.

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Hackaday Links: August 20, 2017

Foam core, dollar tree foam board, Adams foam board, or whatever we’re calling a thin sheet of foam sandwiched between two pieces of poster board, is an invaluable hacker’s tool. Everyone should have a few sheets on hand, and not just because each sheet is a dollar each at any Dollar Store. [Eric] has been working on a technique to create compound curves in foam board, and the results look great. It’s a true three-dimensional plane with weird curves, and certainly has applications for something.

The Apollo Lunar Module is the first, and only manned space-only spacecraft ever made. The design of this spacecraft isn’t constrained by trivialities like ‘atmosphere’, and the design didn’t need ‘bulkheads thicker than a stack of paper towels’. It is a beautiful ship, and now a company wants to produce a gorgeous 1/32 scale model of the LEM. The goal is $25k, which is quite high for the real space modeling market, but if this GoFundMe campaign succeeds, this will be one of the finest real space models ever created. It’ll also match the scale of the 1/32 Revell CSM.

Speaking of Apollo-related technology, here’s a slight bit of drama. [Fran] has been working on recreating the DSKY — the user interface for the Apollo Guidance Computer — for a few years now. She’s set up a crowdfunding campaign to recreate the electroluminescent, screen printed segment display, and things are going great. Now there’s a company selling commercial DSKYs (with a stupid TFT display), that potentially uses the same art. Is this copyright infringement? Maybe, but probably not. It is a dick move not to credit [Fran], though.

The Monoprice Mini Delta is phenomenal. More on that in a bit.

There’s a complete solar eclipse happening across the United States tomorrow. Many schools should have started classes by then, but they’re calling tomorrow a snow day. Everyone who is traveling to see the eclipse is probably already where they’re going to be, and there are clouds on the horizon. Literal clouds. Everyone is watching the weather channel to see what the cloud cover will be tomorrow. Some people don’t have to worry: [Dan] is building a high-altitude balloon to get 100,000 feet above any clouds. There’s a 360° camera onboard, and the resulting video will be awesome. At least one person in Charleston will be renting a plane; I question the wisdom of renting a 172 over a Piper or Cirrus or another low-wing plane, but whatever. If you’re working on a project that will look at the eclipse from above the clouds, leave a note in the comments. For those of you looking at clouds tomorrow, Hackaday is doing another eclipse meet up on the Pacific coast of Mexico on April 8, 2024.

Retrotechtacular: Olivetti Net3

If you sign up for a European hacker camp such as CCC Camp in Germany or SHA Camp in the Netherlands, you’ll see among the items recommended to take with you, a DECT handset. DECT, or Digital Enhanced Cordless Telecommunications, refers to the set of standards that lie behind the digital cordless telephones that are ubiquitous across Europe and some countries elsewhere in the world. These standards cover more than just the simple two-way telephone calls through a base station that most Europeans use them for though, they define a fully functional multi-cell 3G phone and data networking system. This means that an event like SHA Camp can run its own digital phone network without having to implement cell towers.

Olivetti promotional net3 image
Olivetti promotional Net3 image

Reading the history of DECT, there is the interesting snippet that the first DECT product on the market in 1993 was not a telephone but a networking device, and incidentally the first wireless LAN product on the European market. Olivetti’s Net3 provided 512kB/s wireless networking to a base station with Ethernet or Token Ring interfaces for connection to a LAN. In its original form it was an internal card for a desktop PC coupled to a bulky external box containing radio circuitry and antenna, but its later incarnations included a PCMCIA card with a much smaller antenna box. The half-megabit speed seems tiny by today’s standards, but in the pre-multimedia world of 1993 would have been perfectly adequate for a Novell Netware fileserver and an HP Laserjet 4.

Heinz Wolff swallows a condom in another Olivetti promotional picture.
[Heinz Wolff] swallows a condom in another Olivetti promotional image.

Mystery Technology

So DECT is an interesting technology that can do more than just a simple cordless phone, and its first product was unexpectedly somewhat groundbreaking. It then becomes even more interesting to find that Net3 has left very little evidence of itself to find that can be found on the Web, and learning more about it requires a little detective work.

The Wikipedia entry has the bare bones, but it speaks volumes about the obscure nature of the product that the encyclopedia’s only picture of it is a tiny thumbnail-sized promotional image of the PCMCIA variant in a chunky mid-1990s laptop. A further search reveals a 1993 British Olivetti staff newsletter (PDF) carrying another promotional image of the desktop Net3 device featuring the then-well-known TV personality and academic [Heinz Wolff] demonstrating the technology bizarrely by swallowing a DECT medical instrumentation transponder wrapped in a condom. Some press releases remain in the fossilized remnants of the 1990s internet, and a Net3 design team member’s LinkedIn page led us to the patent covering the system, but that’s pretty much it. We can’t even find a high enough resolution image of a Net3 card for our featured image slot.

Wireless Things Before Their Time

It’s obvious that Net3 and DECT networking as a high-end wireless LAN before a need for wireless LANs existed never made it, but what is perhaps more interesting is that it seems to have left no legacy for other more mundane applications. We are in the midst of an explosion of hype around the Internet of Things and it seems new short-range wireless networking technologies appear almost daily, yet the world seems to have overlooked this robust, low power, and mature wireless network with its own dedicated frequency allocation that many of us already have in our homes. It seems particularly surprising that among the many DECT base stations on sale at your local consumer electronics store there are none with an Internet connection, and there is no market for IoT devices that use DECT as their backhaul.

In the open-source community there has been some work on DECT. The OsmocomDECT project for example provides a DECT software stack, and states an aim to “better understand DECT and its security and to create an Open Source implementation of the DECT standard”. But there seems to have been very little hardware work in our community on the standard, for example there are no DECT-specific projects on

Net3 then was a product before its time, a herald of what was to come, from that twilight period when the Web was definitely a thing but had yet to become the world’s universal information repository. Public wireless networking was still several years in the future, so there was no imperative for road warriors to equip themselves with a Net3 card or for computer manufacturers — not even Olivetti themselves! — to incorporate the technology. It thus didn’t take the world by storm, and unusually for such a ground-breaking computer product there remains little legacy for it beyond a rarely-used feature of the protocol Europeans use for their cordless phones.

Did you have a Net3 card? Do you still have one? Let us know in the comments.

Fail of the Week: How Not to Repair a MagSafe Charging Cable

So I made an awful, kludgey, “there I fixed it” level repair, and I need to come clean. This is really a case of an ill-advised ground.

My thirteen-year-old daughter asked for help repairing her Macbook charging cable. Macbook chargers really aren’t meant to flex around a lot, and if you’re the kind of person who uses the laptop on, well, the lap, with the charger in, it’s gonna flex. Sooner or later the insulation around the plug housing, where it plugs into the laptop, cracks and the strands of wire can be seen. This type of cable consists of an insulated lead wire surrounded by a stranded ground wire. The problem with this configuration is that the stranded ground also gets flexed until it breaks, one strand at a time, until the cable stops working.

So it was with my daughter’s Macbook cable. I didn’t have the money to buy her a new one, and I figured we could repair the break. We busted out her WLC100 and sat down to get our solder on. She started off working while I supervised, then I took over later on.

We began by using an Xacto to cut away enough insulation to expose about half an inch of the stranded wire. We pulled the wire away from the insulated lead wire and twisted it into a single stranded wire parallel to the lead wire. Grabbing for the iron, we tinned the ground and soldered a length of 22-gauge solid wire to it. The way the ground connects to the plug is by passing through a conductive ring. My idea was to solder the other end of the 22-gauge wire to the metal ring. Here’s where things started to go wrong. This is, by the way, the part where I took over so you can blame me and not my kid.

My daughter was using the WLC100’s default tip. I should have grabbed my own iron, a WES51, or at least swapped in its ninja-sharp tip. The WLC100’s default tip is a big fat wedge and it was too big to put next to the plug, and the conductive ring quickly got covered in melted plastic and I couldn’t solder anything to it. Worse, I had accidentally burned through the insulation protecting the lead wire, and had to cover it in electrical tape.

iFixit cracked it open and started from scratch.

What now? We were left with not being able to use the cable at all. One option was to wait until the goop had cooled and burnish it clean with a Dremel, then attempt to re-solder using an appropriate tip. However, that sounded like a lot of work. The solid wire was still securely soldered to the ground, so instead of trying to attach it to the cable side of the plug, I could connect it to the computer side, by shoving it into the socket alongside the plug. The business end of the plug has a big silver ground surrounding small gold positive leads, and touching the ground with the wire should work just fine, right?

It did. The computer charged up as happy as you’d like. And yet, I was left with the distinct feeling the solution could have been, I don’t know, cleaner. Certainly, the iFixit route shown here comes out much cleaner by sliding off the housing, clipping the damaged wire, and beginning anew. Clean as this is, it’s just waiting to happen the same way again.

So, brethren and sistren, lay on with brickbats and tell what I did wrong. What approaches have you used to fix cables broken where they meet the plug housing, and how do you improve the situation for the future?

The Best Stereo Valve Amp In The World

There are few greater follies in the world of electronics than that of an electronic engineering student who has just discovered the world of hi-fi audio. I was once that electronic engineering student and here follows a tale of one of my follies. One that incidentally taught me a lot about my craft, and I am thankful to say at least did not cost me much money.

Construction more suited to 1962 than 1992.
Construction more suited to 1962 than 1992.

It must have been some time in the winter of 1991/92, and being immersed in student radio and sound-and-light I was party to an intense hi-fi arms race among the similarly afflicted. Some of my friends had rich parents or jobs on the side and could thus afford shiny amplifiers and the like, but I had neither of those and an elderly Mini to support. My only option therefore was to get creative and build my own. And since the ultimate object of audio desire a quarter century ago was a valve (tube) amp, that was what I decided to tackle.

Nowadays, building a valve amp is a surprisingly straightforward process, as there are many online suppliers who will sell you a kit of parts from the other side of the world. Transformer manufacturers produce readily available products for your HT supply and your audio output matching, so to a certain extent your choice of amp is simply a case of picking your preferred circuit and assembling it. Back then however the world of electronics had extricated itself from the world of valves a couple of decades earlier, so getting your hands on the components was something of a challenge. I cut out the power supply by using a scrap Dymar Electronics instrument enclosure which had built-in HT and heater rails ready to go, but the choice of transformers and high-voltage capacitors was something of a challenge.

Pulling the amplifier out of storage in 2017, I’m going in blind. I remember roughly what I did, but the details have been obscured by decades of other concerns. So in an odd meeting with my barely-adult self, it’s time to take a look at what I made. Where did I get it right, and just how badly did I get it wrong?

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Friday Hack Chat: Graphical Programming Languages with Boian Mitov

There is a long history of Visual or Graphical Programming Languages, and most of them make more sense than the name of Microsoft’s Visual Basic, C#, and Visual Studio IDE. Some people don’t like to code, and for them, graphical programming languages replace semicolons and brackets with easy-to-understand boxes and wires.

This Friday, we’re going to be talking about graphical programming languages with [Boian Mitov]. He’s a software developer, founder of Mitov Software, and the creator of Visuino, a graphical programming language for the embedded domain. Everything from the Arduino to Teensy, ESP8266, ESP32, the chipKIT, and Maple Mini are supported with this IDE. It’s a simple drag-and-drop way of programming microcontrollers that Scratches an itch (see what I did there?) for an easy way to introduce non-programmers to the embedded world and also provides a faster way to build custom applications.

When it comes to graphical programming languages, we can’t find a better Hack Chat guest than [Boian]. He’s the author of the OpenWire dataflow processing technology — another graphical programming language –, the IGDI+ library, VideoLab, SignalLab, AudioLab, PlotLab, InstrumentLab, and author of VCL for Visual C++. He’s a regular contributor to Blaise Pascal Magazine, too.

During this Hack Chat, we’ll be discussing what makes Visual Programming worth it, how and why it works, when it doesn’t and how to develop a graphical programming language. Visuino will be of special interest, And I’m sure someone will work in a, ‘what’s happening with Max/MSP under Ableton’ question. If you have a question for [Boian], here’s a question sheet to guide the discussion.

Here’s How To Take Part:

join-hack-chatOur Hack Chats are live community events on the Hack Chat group messaging. This Hack Chat will take place at noon Pacific time on Friday, August 11th. Here’s a time and date converter!

Log into, visit that page, and look for the ‘Join this Project’ Button. Once you’re part of the project, the button will change to ‘Team Messaging’, which takes you directly to the Hack Chat.

You don’t have to wait until Friday; join whenever you want and you can see what the community is talking about.

Superconference Talk Deadline Extended One Week

Our Call for Proposals for the Hackaday Superconference was scheduled to close yesterday. We are extending that deadline by one week so get your proposal for a talk or a workshop in now.

We want to leave no stone unturned and are intimately familiar with the procrastination habits of busy hackers like you. Now there is no excuse. Put together your pitch now and send it our way. This is the ultimate hardware conference and we’re topics covering Engineering Heroics (how you managed to pull it together to get across the finish line), Prototyping, Research (building custom rigs for University/private industry/giggles), Product Development, Full-Stack Fabrication, and anything else you think fits the vibe of Hackaday.

Accepted talks receive free admission and access to speaker events. There are travel stipends available for exemplary proposals. We also record talks for publication after the Superconference so this is a chance to be famous on Hackaday.

It’s likely that you have an interesting story to tell. Time to get up there and tell it!

The Hackaday SuperConference is November 11-12, 2017 in Pasadena California. There are still tickets available but what remains will sell out quickly when the slate of speakers in announced. Don’t miss out, grab your ticket now.