microscope

109 Articles

Never Drill In The Wrong Place, With This Camera!

June 26, 2023 by 11 Comments

It’s fair to say that one of the biggest advances for the electronic constructor over the last decade or so has been the advent of inexpensive small-order PCB manufacture. That said, there are still plenty who etch their own boards, and for them perhaps the most fiddly part of the process comes in drilling holes accurately. It’s to aid in this task that [John McNelly] has created a camera with a periscope, to give the drill bit perfect alignment with the hole.

The idea is simple enough, an off-the-shelf all-in-one microscope camera points sideways at a mirror allowing it to look upwards. The viewport is placed under the drill and the crosshairs on the microscope are lined up with the end of the drill. Then the board can be placed on top and the pad lined up with the crosshairs, and a perfectly placed hole can be drilled. It’s a beautiful piece of lateral thinking which we like, as it ends that lottery of slightly off-centre holes. You can see it in glorious portrait-mode action in the video below the break.

Oddly this isn’t the first PCB drilling microscope we’ve shown you. but it may well be the more elegant of the two.

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Building An Electron Microscope For Research

April 14, 2023 by 10 Comments

There are a lot of situations where a research group may turn to an electron microscope to get information about whatever system they might be studying. Assessing the structure of a virus or protein, analyzing the morphology of a new nanoparticle, or examining the layout of a semiconductor all might require the use of one of these devices. But if your research involves the electron microscope itself, you might be a little more reluctant to tear down these expensive devices to take a look behind the curtain as the costs to do this for more than a few could quickly get out of hand. That’s why this research group has created their own electron detector.

Specifically, the electron detector is designed for use in a scanning electron microscope, which is typically used for inspecting the surface of a sample and retrieving a high-resolution, 3D image of it compared to transmission microscopes which can probe internal structures. The detector is built on a four-layer PCB which includes the photodiode sensing array, a series of amplifiers, and a power supply. All of the circuit diagrams and schematics are available for inspection as well thanks to the design being licensed under the open Creative Commons license. For any research team looking to build this, a bill of materials is also included, as is a set of build instructions.

While this is only one piece of the puzzle surrounding the setup and operation of an electron microscope, its arguably the most important, and also greatly lowers the barrier of entry for anyone looking to analyze electron microscope design themselves. With an open standard, anyone is free to modify or augment this design as they see fit which is a marked improvement over the closed and expensive proprietary microscopes out there. And, if low-cost microscopes are your thing be sure to check out this fluorescence microscope we featured that uses readily-available parts to dramatically lower the cost compared to commercial offerings.

Glowscope Reduces Microscope Cost By Orders Of Magnitude

March 24, 2023 by 15 Comments

As smartphones become more ubiquitous in society, they are being used in plenty of ways not imaginable even ten or fifteen years ago. Using its sensors to gather LIDAR information, its GPS to get directions, its microphone to instantly translate languages, or even use its WiFi and cellular radios to establish a wireless hotspot are all things which would have taken specialized hardware not more than two decades ago. The latest disruption may be in microscopy, as this build demonstrates a microscope that would otherwise be hundreds of thousands of dollars.

The microscope is a specialized device known as a fluorescence microscope, which uses a light source to excite fluorescent molecules in a sample which can illuminate structures that would otherwise be invisible under a regular microscope. For this build, the light is provided by readily-available LED lighting as well as optical filters typically used in stage lighting, as well as a garden-variety smartphone. With these techniques a microscope can be produced for around $50 USD that has 10 µm resolution.

While these fluorescence microscopes do have some limitations compared to units in the hundred-thousand-dollar range, perhaps unsurprisingly, they are fairly impressive for such a low-cost alternative. More details about these builds can also be found in their research paper published in Nature. Even without the need for fluorescence microscopy, a smartphone has been shown to be a fairly decent optical microscope, provided you have the right hardware to supplement the phone’s camera.

Your Phone Is A 200X Microscope — Sort Of

March 6, 2023 by 19 Comments

[A. Cemal Ekin] over on PetaPixel reviewed the Apexel 200X LED Microscope Lens. The relatively inexpensive accessory promises to transform your cell phone camera into a microscope. Of course, lenses that strap over your phone’s camera lens aren’t exactly a new idea, but this one looks a little more substantial than the usual piece of plastic in a spring-loaded clip. Does it work? You should read [Cemal’s] post for the details, but the answer — as you might have expected — is yes and no.

On the yes side, you can get some pretty neat photomicrographs from the adapter. On the negative side, your phone isn’t made to accommodate microscope samples. It also isn’t made to stay stable at 200X.

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Behold A Microscope That Sees By Squashing Things Into It

February 5, 2023 by 18 Comments

“Look with your eyes, not your hands” is something many of us have heard while growing up, but that doesn’t apply to the touch-sensitive microscope [Steve Mould] got to play with.

Gel pad removed, exposing lens and multi-directional lighting.

The wand-like device is made by Gelsight, and instead of an optical lens like a normal microscope, it sports a gel pad on the sensing end. By squashing an object into the gel, the device is able to carefully illuminate and image the impression created. By taking multiple images lit from different angles, a lot of information can be extracted.

The result is a high-resolution magnification — albeit a monochromatic one — that conveys depth extremely well. It’s pretty neat clearly seeing tiny specks of dust or lint present on surfaces when [Steve] demonstrates imaging things like coin cells.

Many a hacker knows that the devil is in the details when it comes to executing an idea. Even so, the basic principles of the Gelsight seem simple enough and possibly within the realm of inspired DIY in the same way that we saw a CNC gantry and USB microscope repurposed as an optical comparator.

Watch the Gelsight in action in the video below, embedded below the page break.

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Reading Data From A CD, With A Microscope

January 22, 2023 by 18 Comments

There was a time when electronic engineering students studied the audio CD, for all its real-world examples of error correction and control systems. There’s something to be found in the system still for young and old though, and thus we were intrigued when we saw [Peter Monta] reading the data from a CD using a microscope.

CDs encode data as so-called pits and lands in a spiral track across a metalised surface, with a transition from pit to land signifying a logic 1 and a missing transition signifying a 0. Reading a section of the raw data is achieved in the first part of his write-up, but in the next installment he goes further into retrieving more data through stitching together microscope pictures and writing some code to retrieve data frames. He’s not quite at the audio playback stage, but he’s planning in the future to spiral-track a full image to rip an entire disc.

There are plenty of CD drives around to read audio the conventional way, but the techniques here still find a use where less ubiquitous media has to be read. In the last decade for example there was an effort to read the BBC Domesday Project from the 1980s, as it became clear that few of the original readers survived in working order.

Laser Scanning Microscope Built With Blu-ray Parts

December 23, 2022 by 14 Comments

Laser scanning microscopes are useful for all kinds of tiny investigations. As it turns out, you can build one using parts salvaged from a Blu-ray player, as demonstrated by [Doctor Volt].

The trick is repurposing the optical pickup unit that is typically used to read optical discs. In particular, the build relies on the photodiodes that are usually used to compute focus error when tracking a disc. To turn this into a laser scanning microscope, the optical pickup is fitted to a 3D printed assembly that can slew it linearly for imaging purposes.

Meanwhile, the Blu-ray player’s hardware is repurposed to create a sample tray that slews on the orthogonal axis for full X-Y control. An ESP32 is then charged with running motion control and the laser. It also captures signals from the photodiodes and sends them to a computer for collation and display.

[Doctor Volt] demonstrates the microscope by imaging a small fabric fragment. The scanned area covers less than 1 mm x 1 mm, with a resolution of 127 x 127, though this could be improved with finer pitch on the slew mechanisms.

While it’s hardly what we’d call a beginner’s project, this technique still looks a lot more approachable than building your own scanning electron microscope.

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