How Peptides Are Made

What does body building, anti-aging cream and Bleomycin (a cancer drug) have in common? Peptides of course! Peptides are large molecules that are vital to life. If you were to take a protein and break it into smaller pieces, each piece would be called a peptide. Just like proteins, peptides are made of amino acids linked together in a chain-like structure. Whenever you ingest a protein, your body breaks it down to its individual amino acids. It then puts those amino acids back together in a different order to make whatever peptide or protein your body needs. Insulin, for instance, is a peptide that is 51 amino acids long. Your body synthesizes insulin from the amino acids it gets from the proteins you eat.

Peptides and small proteins can be synthesized in a lab as well. Peptide synthesis is a huge market in the pharmaceutical and skin care industry. They’re also used, somewhat shadily, as a steroid substitute by serious athletes and body builders. In this article, we’re going to go over the basic steps of how to join amino acids together to make a peptide. The chemistry of peptide synthesis is complex and well beyond the scope of this article. But the basic steps of making a peptide are not as difficult as you might think. Join me after the break to gain a basic understanding of how peptides are synthesized in labs across the world, and to establish a good footing should you ever wish to delve deeper and make peptides on your own.

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The Enigma Enigma: How The Enigma Machine Worked

To many, the Enigma machine is an enigma. But it’s really quite simple. The following is a step-by-step explanation of how it works, from the basics to the full machine.

Possibly the greatest dedicated cipher machine in human history the Enigma machine is a typewriter-sized machine, with keyboard included, that the Germans used to encrypt and decrypt messages during World War II. It’s also one of the machines that the Polish Cipher Bureau and those at Britain’s Bletchley Park figured out how to decipher, or break. Most recently the story of how it was broken was the topic of the movie The Imitation Game.

Let’s start with the basics.

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Living Logic: Biological Circuits For The Electrically Minded

Did you know you can build fundamental circuits using biological methods? These aren’t your average circuits, but they work just like common electrical components. We talk alot about normal silicon and copper circuits ‘roud here, but it’s time to get our hands wet and see what we can do with the power of life!

In 1703, Gottfried Wilhelm Leibniz published his Explication de l’Arithmétique Binaire (translated). Inspired by the I Ching, an ancient Chinese classic, Leibniz established that the principles of arithmetic and logic could be combined and represented by just 1s and 0s. Two hundred years later in 1907, Lee De Forest’s “Audion” is used as an AND gate. Forty years later in 1947, Brattain and H. R. Moore demonstrate their “PNP point-contact germanium transistor” in Bell Labs (often given as the birth date of the transistor). Six years later in 1953, the world’s first transistor computer was created by the University of Manchester. Today, 13,086,801,423,016,741,282,5001 transistors have built a world of progressing connectivity, automation and analysis.

While we will never know how Fu Hsi, Leibniz, Forest or Moore felt as they lay the foundation of the digital world we know today, we’re not completely out of luck: we’re in the midst’s of our own growing revolution, but this one’s centered around biotechnology. In 1961, Jacob and Monod discovered the lac system: a biological analog to the PNP transistor presented in Bell Labs fourteen years earlier. In 2000, Gardner, Cantor, and Collins created a genetic toggle switch controlled by heat and a synthetic fluid bio-analog2. Today, AND, OR, NOR, NAND, and XOR gates (among others) have been successfully demonstrated in academic labs around the world.

But wait a moment. Revolution you say? Electrical transistors went from invention to computers in 6 years, and biological transistors went from invention to toggle button in 40? I’m going to get to the challenges facing biological circuits in time, but suffice it to say that working with living things that want to be fed and (seem to) like to die comes with its own set of challenges that aren’t relevant when working with inanimate and uncaring transistors. But, in the spirit of hacking, let’s dive right in. Continue reading “Living Logic: Biological Circuits For The Electrically Minded”

Monoprice Mini Delta Review

For the last year or so, Monoprice has been teasing their follow-up to the fantastic $200 MP Select Mini. This is the $150 mini delta printer. We got a look at it last January at CES, it was on display at the Bay Area Maker Faire last May. Now there’s one on the Hackaday review desk.

Over the last few years, 3D printing has settled down into what most of us expected way back in 2010. No, not everyone wants, or arguably needs, a 3D printer on their desks. This is a far cry from the hype of a few years ago, leaving us with what we have today. 3D printers are just tools, much like a drill press or a laser cutter.

With that said, there still are some fantastic advances in 3D printing coming down from on high. Prusa will be shipping the 4-color multi-extruder add-on for the i3 Mk 2 shortly, and somehow or another we have infinite build volume printers. Still, there’s space to democratize 3D printing, and an opportunity for someone to release a very cheap, very good printer.

Monoprice was kind enough to send me a review unit of the MP Mini Delta before it officially hit their website. This is one of the first off the production line, alongside the few hundred pre ordered on an Indiegogo campaign earlier this year.  Does this printer live up to expectations? It sure does, and that’s not just because it’s a $150 printer.

This would be an excellent printer at three times the price, and evidence enough that 3D printing is changing from a weird hobbyist thing to a proper tool.

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Living On Mars: The Stuff You Never Thought About

In The Martian we saw what kind of hacking was needed to stay alive for a relatively short while on Mars, but what if you were trying to live there permanently? Mars’ hostile environment would affect your house, your transportation, even how you communicate. So here’s a fun thought experiment about how you’d live on Mars as part of a larger community.

Not Your Normal House

Mars One living units under regolith
Mars One living units under regolith, Source video

Radiation on Mars comes from solar particle events (SPE) and galactic cosmic radiation (GCR). Mars One, the organization planning one-way trips to Mars talks about covering their habitats in several meters of regolith, a fancy word for the miscellaneous rocky material covering the bedrock. Five meters provides the same protection as the Earth’s atmosphere — around 1,000 g/cm2 of shielding. A paper from the NASA Langley Research Center says that the largest reduction comes from the top 15 to 20 cm of regolith. And so our Mars house will have an underlying structure but the radiation protection will come from somewhere between 20 cm to a few meters of regolith. Effectively, people will be living underground.

On Earth, producing water and air for your house is not something you think of doing, let alone disposing of exhaled CO2. But Mars houses will need systems for this and more.

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Product Development And Avoiding Stock Problems

You’ve spent months developing your product, your Kickstarter just finished successfully, and now you’re ready to order all the parts. Unfortunately, your main component, an ATmega328P, is out of stock everywhere with a manufacturer lead time of 16 weeks. Now what?

When manufacturing things in large volumes, acquiring enough stock at the right time can be tricky. There can be seasonal shortages with companies trying to get products manufactured and available for Christmas. There can be natural disasters like floods of hard drive factories, or politically-related availability problems like tantalum for capacitors, or maybe new markets open up that increase demand or a new product sucks up all the available supply. The result is all the same; you have a harder time getting what you need. Fortunately, there are some ways to avoid this problem, or at least mitigate it.

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Hands On With The SHACamp 2017 Badge

The badge has become one of the defining features of a modern hacker camp, a wearable electronic device that serves as both event computer and platform for some mild software and hardware hacking. Some events have had astoundingly sophisticated badges while others are more simple affairs, and the phenomenon has even spawned an ecosystem of unofficial badges which have nothing to do with the event in question.

The SHACamp 2017 badge is the latest to come the way of a Hackaday writer, and certainly contains enough to be taken as representative of the state of hacker camp badges in 2017. It doesn’t have a star turn like CCCCamp 2015’s software defined radio, instead it’s an extremely handy little computer in its own right.

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