Too much of a good thing can be a bad thing, and nitrate pollution due to agricultural fertilizer runoff is a major problem for both lakes and coastal waters. Assessing nitrate levels commercially is an expensive process that uses proprietary instruments and toxic reagents such as cadmium. But [Joshua Pearce] has recently developed an open-source photometer for nitrate field measurement that uses an enzyme from spinach and costs a mere $65USD to build.
The device itself is incredibly simple – a 3D printed enclosure houses an LED light source and a light sensor. The sample to be tested is mixed with a commercially available reagent kit based on the enzyme nitrate reductase, resulting in a characteristic color change proportional to the amount of nitrate present. The instrument reads the amount of light absorbed by the sample, and communicates the results to an Android device over a Bluetooth link.
Open-source instruments like this can really open up educational opportunities for STEM groups to get out into the real world and start making measurements that can make a difference. Not only can this enable citizen scientists and activists, but it also opens the door for getting farmers involved in controlling nitrate pollution at its source – knowing when a field has been fertilized enough can save a farmer unnecessary expense and reduce nitrate runoff.
There are a lot of other ways to put an open-source instrument like this to use in biohacking – photometery is a very common measuring modality in the life sciences, after all. We’ve seen similar instruments before, like a DIY spectrophotometer, or this 2015 Hackaday Prize entry medical tricorder with a built-in spectrophotometer. Still, for simplicity of build and potential impact, it’s hard to beat this instrument.





Standing only about 16 cm tall, the cabinet is quite detailed and resembles the familiar arcade form factor that has consumed countless quarters. It even appears to be made of particle board like the big boys. The screen cutout is filled by a 84×48 monochrome Nokia display, and the rest of the cabinet’s interior is stuffed with a CNC-milled PCB, temperature and humidity sensors, an RTC, and a Bluetooth module for uploading data to a phone. [Rui] even manages to work in an homage to the grand-daddy of all arcade games with a Pong splash screen.
