On-Site Viral RNA Detection In Wastewater With Paper And Wax Microfluidics

October 9, 2024 by Maya Posch 4 Comments

Schematic version of on-site wastewater analysis using the microfluidic strips (Credit: Yuwei Pan et al., Cell, 2024)

Wastewater sampling has become a popular way over the years to keep track of the health of a population, including human ones, as pathogens are often detectable in the effluence from toilets. Since most houses connected to the centralized sewer systems, this means that a few sampling sites suffice to keep tabs on which viruses are circulating in an area. While sampling this wastewater is easy, the actual RNA analysis using PCR (polymerase chain reaction) still has to be performed in laboratories, adding complex logistics. An approach for on-site analysis using microfluidics was tested out by [Yuwei Pan] et al., as recently published in Cell.

This particular approach uses RT-LAMP (reverse-transcription loop-mediated isothermal amplification) to increase the amount of genetic material, which has the significant benefit over PCR that it does not require multiple thermal cycles, instead being run at a constant temperature. The filter paper used as the basis has wax microchannels printed on it, which help to guide the filtered wastewater to the reaction chambers. This is in many ways reminiscent of the all too familiar linear flow self-tests (RAT: rapid antigen test) that have become one of the hallmarks of the SARS-CoV-2 pandemic.

What this paper microfluidic device adds is that it doesn’t merely contain antigens, but performs the lysis (i.e. breakdown of the virus particles), genetic material multiplication using RT-LAMP and subsequent presence detection of certain RNA sequences to ascertain the presence of specific viruses. Having been used in the field already since 2020 in the UK, the researchers envision this type of on-site analysis to be combined with a smartphone for instant recording and transmission to health authorities.

Some of the benefits of this approach would be lower cost, easier logistics and faster results compared to shipping wastewater samples to central laboratories.

3D Printed Bearings With Filament Rollers

October 9, 2024 by Danie Conradie 24 Comments

Commodity bearings are a a boon for makers who to want something to rotate smoothly, but what if you don’t have one in a pinch? [Cliff] of might have the answer for you, in the form of 3D printed bearings with filament rollers.

With the exception of the raw filament rollers, the inner and outer race, roller cage and cap are all printed. It would also be possible to design some of the components right into a rotating assembly. [Cliff] makes it clear this experiment isn’t about replacing metal bearings — far from it. Instead, it’s an inquiry into how self-sufficient one can be with a FDM 3D printer. That didn’t stop him from torture testing the design to its limits as wheel bearings on an off-road go-cart. The first version wasn’t well supported against axial loads, and ripped apart during some more enthusiastic maneuvers.

[Cliff] improved it with a updated inner race and some 3D printed washers, which held up to 30 minutes of riding with only minimal signs of wear. He also made a slightly more practical 10 mm OD version that fits over an M3 bolt, and all the design files are downloadable for free. Cutting the many pieces of filament to length quickly turned into a chore, so a simple cutting jig is also included.

Let us know in the comments below where you think these would be practical. We’ve covered some other 3D printed bearing that use printed races, as well as a slew bearing that’s completely printed. Continue reading “3D Printed Bearings With Filament Rollers” →

Fail Of The Week: The Case Of The Curiously Colored Streetlights

October 9, 2024 by Dan Maloney 119 Comments

What color are the street lights in your town? While an unfortunate few still suffer under one of the awful colors offered by vapor discharge lamps, like the pink or orange of sodium or the greenish-white of mercury, most municipalities have moved to energy-saving LED streetlights, with a bright white light that’s generally superior in every way. Unless, of course, things go wrong and the lights start to mysteriously change colors.

If you’ve noticed this trend in your area, relax; [NanoPalomaki] has an in-depth and surprisingly interesting analysis of why LED streetlights are changing colors. After examining a few streetlights removed from service thanks to changing from white to purple, he discovered a simple explanation. White LEDs aren’t emitting white light directly; rather, the white light comes from phosphors coating the underlying LED, which emits a deep blue light. The defunct units all showed signs of phosphor degradation. In some cases, the phosphors seemed discolored, as if they experienced overheating or chemical changes. In other LEDs the phosphor layer was physically separated from the backing, exposing the underlying LEDs completely. The color of these damaged modules was significantly shifted toward the blue end of the spectrum, which was obviously why they were removed from service.

Now, a discolored LED here and there does not exactly constitute a streetlight emergency, but it’s happening to enough cities that people are starting to take notice. The obvious solution would be for municipalities to replace the dodgy units Even in the unlikely event that a city would get some compensation from the manufacturer, this seems like an expensive proposition. Luckily, [NanoPalomaki] tested a solution: he mixed a wideband phosphor into a UV-curable resin and painted it onto the lens of each defective LED in the fixture. Two coats seemed to do the trick.

We have to admit that we have a hard time visualizing a city employee painstakingly painting LEDs when swapping out a fixture would take an electrician a few minutes, but at least it’s an option. And, it’s something for hobbyists and homeowners faced with the problem of wonky white LEDs to keep in mind too.

Continue reading “Fail Of The Week: The Case Of The Curiously Colored Streetlights” →

FLOSS Weekly Episode 804: The AI Alliance — Asimov Was Right

October 9, 2024 by Jonathan Bennett 1 Comment

This week Jonathan Bennett and and Dan Lynch chat with Anthony Annunziata about Open Source AI and the AI Alliance. We get answers to our burning AI questions, and talk about the difficulty of defining what Open Source means for these large models.

Continue reading “FLOSS Weekly Episode 804: The AI Alliance — Asimov Was Right” →

Braun TS2 Radio Turns 68, Gets Makeover

October 9, 2024 by Al Williams 7 Comments

The Braun TS2 radio was a state-of-the art tube set in 1956. Today it still looks great, but unsurprisingly, the one that [Manuel Caldeier] has needed a little tender loving care. The table radio had a distinct style for its day and push-buttons. However, the dial glass and the speaker grill needed replacement. Even more interesting, the radio has a troublesome selenium rectifier, giving him the perfect chance to try out his new selenium rectifier solid-state replacement.

The radio is as good-looking inside as it is outside. You can tell that this isn’t his first restoration, as he has several tricks to test things at different stages of the project.

While the radio looked good, it smelled of smoke, which required a big effort to clean. The dial glass was intact enough for him to duplicate it in a graphic program and print it on a transparent adhesive sticker. With a deep breath, he removed the original markings from the glass so he could add the sticker to it. That didn’t work because the label needed cutouts. So now he is waiting for a piece of acrylic that will have the art UV printed on it.

We want to see the next part as we imagine the radio sounds as good as it looks when it is working. If you want to know more about the rectifier replacement, we covered that earlier. Even years later, Braun would have a clean aesthetic.

Continue reading “Braun TS2 Radio Turns 68, Gets Makeover” →

Lagrange Points And Why You Want To Get Stuck At Them

October 9, 2024 by Maya Posch 35 Comments

Visualization of the Sun-Earth Lagrange points.

Orbital mechanics is a fun subject, as it involves a lot of seemingly empty space that’s nevertheless full of very real forces, all of which must be taken into account lest one’s spacecraft ends up performing a sudden lithobraking maneuver into a planet or other significant collection of matter in said mostly empty space. The primary concern here is that of gravitational pull, and the way it affects one’s trajectory and velocity. With a single planet providing said gravitational pull this is quite straightforward to determine, but add in another body (like the Moon) and things get trickier. Add another big planetary body (or a star like our Sun), and you suddenly got yourself the restricted three-body problem, which has vexed mathematicians and others for centuries.

The three-body problem concerns the initial positions and velocities of three point masses. As they orbit each other and one tries to calculate their trajectories using Newton’s laws of motion and law of universal gravitation (or their later equivalents), the finding is that of a chaotic system, without a closed-form solution. In the context of orbital mechanics involving the Earth, Moon and Sun this is rather annoying, but in 1772 Joseph-Louis Lagrange found a family of solutions in which the three masses form an equilateral triangle at each instant. Together with earlier work by Leonhard Euler led to the discovery of what today are known as Lagrangian (or Lagrange) points.

Having a few spots in an N-body configuration where you can be reasonably certain that your spacecraft won’t suddenly bugger off into weird directions that necessitate position corrections using wasteful thruster activations is definitely a plus. This is why especially space-based observatories such as the James Webb Space Telescope love to hang around in these spots.

Continue reading “Lagrange Points And Why You Want To Get Stuck At Them” →

Dot-Matrix Printer Brings Old School Feel To Today’s Headlines

October 9, 2024 by Dan Maloney 20 Comments

If you remember a time when TV news sets universally incorporated a room full of clattering wire service teleprinters to emphasize the seriousness of the news business, congratulations — you’re old. Now, most of us get our news piped directly into our phones, selected by algorithms perfectly tuned to rile us up on whatever the hot-button issue du jour happens to be. Welcome to the future.

If like us you long for a simpler way to get your news, [Andrew Schmelyun] has a partial solution with this dot-matrix news feeder. It’s part of his effort to detox a bit from the whole algorithm thing and make the news a little more concrete. He managed to chase down a very old Star Micronics printer with a serial interface, which he got on the cheap thanks to the previous owner not being sure if it worked. It did, at least after some cleaning, and thanks to a USB-to-serial and the efforts of Linux kernel hackers through the ages, was able to echo output to the printer from a Raspberry Pi Zero W.

From there, getting a daily news feed was as simple as writing some PHP code to mine the APIs of a few selected services. We’re perplexed and alarmed to report that Hackaday is not among the selected sources, but we’re sure this was just a small oversight that will be corrected in version 2. The program runs as a cron job so that a dead-tree version of the day’s top stories is ready for [Andrew]’s morning coffee.

We’ve seen similar news printers before; we particularly like this roll-feed paper version. But for a seriously retro feel, we’d love to see this done on a real teletype.