A researcher in a safety harness pollinates an American chestnut tree from a lift. Another researcher is on the other side of the lift and appears to be taking notes. The tree has bags over some of its branches, presumably to control the pollen that gets in. The lift has a grey platform and orange arm.

Hacking Trees To Bring Back The American Chestnut

“Chestnuts Roasting on an Open Fire” is playing on the radio now in the Northern Hemisphere which begs the question, “What happened to the American chestnut?” Would you be surprised to hear there’s a group dedicated to bringing it back from “functional extinction?” [via Inhabitat]

Between logging and the introduction of chestnut blight, the once prevalent American chestnut became increasingly uncommon throughout its traditional range in the Appalachians. While many trees in the southern range were killed by Phytophthora root rot (PRR), the chestnut blight leaves roots intact, so many chestnuts have been surviving by growing back from the roots only to succumb to the blight and be reborn again. Now, scientists are using a combination of techniques to develop blight-resistant trees from this remaining population.

The American Chestnut Foundation recognizes you can’t improve what you can’t measure and uses a combination of “small stem assays (SSAs) performed on potted seedlings, improved phenotype scoring methods for field-grown trees, and the use of genomic prediction models for scoring resistance based on genotype.” This allows them to more rapidly screen varieties for blight resistance to further their efforts. One approach is based on conventional plant breeding techniques and has been crossing blight and PRR-resistant Chinese chestnuts with the American type. PRR resistance has been found to be less genetically complicated, so progress has been faster on resistance to that particular problem. Continue reading “Hacking Trees To Bring Back The American Chestnut”

A cartoon of the Sun above a windmill and a solar panel with a lightning bolt going to a big grey gear with "AAAp" written on it. A small "e-" on a circle is next to it, indicating electricity transfer. Further to the right is an ADP molecule connected to a curved arrow going through the AAAp gear to turn into ATP. Three cartoon shapes, presumably illustrating biological processes are on the right with arrows pointing from the ATP.

Powering Biology With Batteries

We’ve all been there — you forgot your lunch, but there are AC outlets galore. Wouldn’t it be so much simpler if you could just plug in like your phone? Don’t try it yet, but biologists have taken us one step further to being able to fuel ourselves on those sweet, sweet electrons.

Using an “electrobiological module” of 3-4 enzymes, the amusingly named AAA (acid/aldehyde ATP) cycle regenerates ATP in biological systems directly from electricity. The process takes place at -0.6 V vs a standard hydrogen electrode (SHE), and is compatible with biological transcription/translation processes like “RNA and protein synthesis from DNA.”

The process isn’t dependent on any membranes to foul or more complicated sets of enzymes making it ideal for in vitro synthetic biology since you don’t have to worry about keeping as many components in an ideal environment. We’re particularly interested in how this might apply to DNA computing which we keep being promised will someday be the best thing since the transistor.

Maybe in the future we’ll all jack in instead of eating our daily food pill? If this all seems like something you’ve heard of before, but in reverse, maybe you’re thinking of microbial fuel cells.

Optical Tweezers Investigate Tiny Particles

No matter how small you make a pair of tweezers, there will always be things that tweezers aren’t great at handling. Among those are various fluids, and especially aerosolized droplets, which can’t be easily picked apart and examined by a blunt tool like tweezers. For that you’ll want to reach for a specialized tool like this laser-based tool which can illuminate and manipulate tiny droplets and other particles.

[Janis]’s optical tweezers use both a 170 milliwatt laser from a DVD burner and a second, more powerful half-watt blue laser. Using these lasers a mist of fine particles, in this case glycerol, can be investigated for particle size among other physical characteristics. First, he looks for a location in a test tube where movement of the particles from convective heating the chimney effect is minimized. Once a favorable location is found, a specific particle can be trapped by the laser and will exhibit diffraction rings, or a scattering of the laser light in a specific way which can provide more information about the trapped particle.

Admittedly this is a niche tool that might not get a lot of attention outside of certain interests but for those working with proteins, individual molecules, measuring and studying cells, or, like this project, investigating colloidal particles it can be indispensable. It’s also interesting how one can be built largely from used optical drives, like this laser engraver that uses more than just the laser, or even this scanning laser microscope.

Continue reading “Optical Tweezers Investigate Tiny Particles”

Simulating Cellular Biology In The Browser

[Technistuff] read a paper about simulating a “minimal” cell — apparently a cell with only 493 genes. This led to a goal: reproduce the simulation in TypeScript so it can run in a web browser. Why? We don’t know, but it is an interesting look at both in-depth biology and how to handle complex simulations. The code is available on GitHub.

For a point of reference, E. Coli has over 4,500 genes. The cell in question — JCVI-syn3A — actually has seven more genes than truly necessary. The data for this bacteria is available from a research lab, again, using GitHub.

Continue reading “Simulating Cellular Biology In The Browser”

a) Schematic illustration of energy storage process of succulent plants by harnessing solar energy with a solar cell, and the solar cell converts the energy into electricity that can be store in APCSCs of succulent plants, and then utilized by multiple electrical appliances. b–d) The energy is stored in cactus under sunlight by solar cell and then power light strips of Christmas tree for decoration.

Succulents Into Supercapacitors

Researchers in Beijing have discovered a way to turn succulents into supercapacitors to help store energy. While previous research has found ways to store energy in plants, it often required implants or other modifications to the plant itself to function. These foreign components might be rejected by the plant or hamper its natural functions leading to its premature death.

This new method takes an aloe leaf, freeze dries it, heats it up, then uses the resulting components as an implant back into the aloe plant. Since it’s all aloe all the time, the plant stays happy (or at least alive) and becomes an electrolytic supercapacitor.

Using the natural electrolytes of the aloe juice, the supercapacitor can then be charged and discharged as needed. The researchers tested the concept by solar charging the capacitor and then using that to run LED lights.

This certainly proposes some interesting applications, although we think your HOA might not be a fan. We also wonder if there might be a way to use the photosynthetic process more directly to charge the plant? Maybe this could recharge a tiny robot that lands on the plants?

Some Bacteria Could Have A Rudimentary Form Of Memory

When we think of bacteria, we think of simple single-celled organisms that basically exist to consume resources and reproduce. They don’t think, feel, or remember… or do they? Bacteria don’t have brains, and as far as we know, they’re incapable of thought. But could they react to an experience and recall it later?

New research suggests that some bacteria could have a rudimentary form of memory of their experiences in the environment. They could even pass this memory down across generations via a unique mechanism. Let’s dive into the latest research that is investigating just what bacteria know, and how they happen to know it.

Continue reading “Some Bacteria Could Have A Rudimentary Form Of Memory”

Exploring Tropical Rainforest Stratification Using Space-Based LiDAR

GEDI is deployed on the the Japanese Experiment Module – Exposed Facility (JEM-EF). The highlighted box shows the location of GEDI on the JEM-EF.
GEDI is deployed on the the Japanese Experiment Module – Exposed Facility (JEM-EF). The highlighted box shows the location of GEDI on the JEM-EF.

Even though it may seem like we have already explored every single square centimeter of the Earth, there are still many areas that are practically unmapped. These areas include the bottom of the Earth’s oceans, but also the canopy of the planet’s rainforests. Rather having herds of explorers clamber around in the upper reaches of these forests to take measurements, researchers decided to use LiDAR to create a 3D map of these forests (press release).

The resulting GEDI (Global Ecosystem Dynamics Investigation) NASA project includes a triple-laser-based LiDAR system that was launched to the International Space Station in late 2018 by CRS-16 where it has fulfilled its two-year mission which began in March of 2019. Included in the parameters recorded this way are surface topography, canopy height metrics, canopy cover metrics and vertical structure metrics.

Originally, the LiDAR scanner was supposed to be decommissioned by stuffing it into the trunk of a Dragon craft before its deorbit, but after NASA found a way to scoot the scanner over to make way for a DOD payload, the project looks to resume scanning the Earth’s forests next year, where it can safely remain until the ISS is deorbited in 2031. Courtesy of the ISS’s continuous orbiting of the Earth, it’ll enable daily monitoring of its rainforests in particular, which gives us invaluable information about the ecosystems they harbor, as well as whether they’re thriving or not.

Hopefully after its hibernation period the orbital LiDAR scanner will be back in action, as the instrument is subjected to quite severe temperature changes in its storage location. Regardless, putting LiDAR scanners in orbit has to be one of those amazing ideas to help us keep track of such simple things as measuring the height of trees and density of foliage.