Lego Microscope Aims To Discover Future Scientists

When it comes to inspiring a lifelong appreciation of science, few experiences are as powerful as that first glimpse of the world swimming in a drop of pond water as seen through a decent microscope. But sadly, access to a microscope is hardly universal, denying that life-changing view of the world to far too many people.

There have been plenty of attempts to fix this problem before, but we’re intrigued to see Legos used to build a usable microscope, primarily for STEM outreach. It’s the subject of a scholarly paper (preprint) by [Bart E. Vos], [Emil Betz Blesa], and [Timo Betz]. The build almost exclusively uses Lego parts — pretty common ones at that — and there’s a complete list of the parts needed, which can either be sourced from online suppliers, who will kit up the parts for you, or by digging through the old Lego bin. Even the illuminator is a stock part, although you’ll likely want to replace the orange LED buried within with a white one. The only major non-Lego parts are the lenses, which can either be sourced online or, for the high-power objective, pulled from an old iPhone camera. The really slick part is the build instructions (PDF), which are formatted exactly like the manual from any Lego kit, making the build process easily accessible to anyone who has built Lego before.

As for results, they’re really not bad. Images of typical samples, like salt crystal, red onion cells, and water fleas are remarkably clear and detailed. It might no be a lab-grade Lego microscope, but it looks like it’s more than up to its intended use.

Thanks for the heads up on this, [Jef].

Thin Coatings Require An Impressive Collection Of Equipment And Know-How

Let’s be honest — not too many of us have a need to deposit nanometer-thick films onto substrates in a controlled manner. But if you do find yourself in such a situation, you could do worse than following [Jeroen Vleggaar]’s lead as he builds out a physical vapor deposition apparatus to do just that.

Thankfully, [Jeroen] has particular expertise in this area, and is willing to share it. PVD is used to apply an exceedingly thin layer of metal or organic material to a substrate — think lens coatings or mirror silvering, as well as semiconductor manufacturing. The method involves heating the coating material in a vacuum such that it vaporizes and accumulates on a substrate in a controlled fashion. Sounds simple, but the equipment and know-how needed to actually accomplish it are daunting. [Jeroen]’s shopping list included high-current power supplies to heat the coating material, turbomolecular pumps to evacuate the coating chamber, and instruments to monitor the conditions inside the chamber. Most of the chamber itself was homemade, a gutsy move for a novice TIG welder. Highlights from the build are in the video below, which also shows the PVD setup coating a glass disc with a thin layer of silver.

This build is chock full of nice details; we especially liked the technique of monitoring deposition progress by measuring the frequency change of an oscillator connected to a crystal inside the chamber as it accumulates costing material. We’re not sure where [Jeroen] is going with this, but we suspect it has something to do with some hints he dropped while talking about his experiments with optical logic gates. We’re looking forward to seeing if that’s true.

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The Laser Power Record Has Been Broken

Lasers do all sorts of interesting things and — as with so many things — more is better. Korean scientists announced recently they’ve created the most powerful laser beam. 1023 watts per square centimeter, to be exact. It turns out that 1022 Watts/cm2 may not be commonplace, but has been done many times already at several facilities, including the CoReLS petawatt (PW) laser used by the researchers.

Just as improving a radio transmitter often involves antenna work instead of actual power increases, this laser setup uses an improved focus mechanism to get more energy in a 1.1 micron spot. As you might expect, doing this requires some pretty sophisticated optics.

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Coffee Stirrers Act As Lens For This Digital Straw Camera

What happens when you mix over 23,000 coffee stirrers and a Raspberry Pi camera together? Probably nothing except for a mess, unless you very specifically pack the plastic straws and orient the camera just right. In that case, you get this very cool lenless digital straw camera that takes artfully ghostly images.

Image of Yoda photographed through many straws

Actually, lensless is a bit of a reach for [Adrian Hanft]’s creation. While the camera he’s using to grab the image has a lens, the objective, for lack of a better term, is just a tightly packed bundle of straws. We’ve seen this approach to photography before, but there the camera used film placed at the back of the straw bundles to capture the pixelated image.

Here, a ground glass screen stands in for the film; a long lightproof box behind that provide a place to mount a camera to capture the images. Cleverly, [Adrian] built the camera mount from Lego, allowing cameras and lenses to be quickly swapped out. A Nintendo gamepad controller talks to custom software running on a Raspberry Pi and allows the photographer to control exposure and scroll through pictures using a smartphone as a display. There’s a short build video below, for those who can’t get enough of straw-packing techniques.

As with the film version of this camera, we just love the look of the photographs that come from this — the texture of the straw honeycomb and the defocused subject make for a striking effect.

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3D-Printed Laser Scanning Confocal Microscope Measures Microns

When one thinks about microscopy, it seems to be mostly qualitative. Looking at a slide teeming with bacteria or protozoans is less about making measurements and more about recognizing features and describing their appearance. Not all microscopes are created equal, though, with some being far more optimized for making fine measurements of the microscopic realm.

This 3D-printed confocal laser scanning microscope is a good example of an instrument for measuring really small stuff. As [Zachary Tong] points out, confocal scanning microscopy uses a clever optical setup to collect light from a single, well-defined point within a sample; rather than getting an image of all the points within a two-dimensional focal plane, the scanning function moves the focal point around through the sample in three dimensions, capturing spatial data to go along with the optical information.

The stage of [Zach]’s microscope is based on OpenFlexure’s Delta Stage, an open-source, 3D-printed delta-bot motion control platform that’s capable of positioning samples with sub-micron precision. Above the stage are the deceptively simple optics, with a laser diode light source, an objective lens, and a photodiode detector behind a pinhole. The detector feeds a homebrew trans-impedance amplifier that captures data at millions of points as the sample is moved through a small three-dimensional space. All that data gets crunched to find the Z-axis position corresponding to the maximum intensity at each point.

It takes a while to gather all this data — up to several days for even a small sample — but it works pretty well. [Zach] already has some ideas for reducing noise and speeding up the scan time; perhaps a stage based on DVD parts like this one would be faster than the delta stage. We look forward to seeing his improvements.

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Pi-Based Spectrometer Puts The Complexity In The Software

Play around with optics long enough and sooner or later you’re probably going to want a spectrometer. Optical instruments are famously expensive, though, at least for high-quality units. But a useful spectrometer, like this DIY Raspberry Pi-based instrument, doesn’t necessarily have to break the bank.

This one comes to us by way of [Les Wright], whose homebrew laser builds we’ve been admiring for a while now. [Les] managed to keep the costs to a minimum here by keeping the optics super simple. The front end of the instrument is just a handheld diffraction-grating spectroscope, of the kind used in physics classrooms to demonstrate the spectral characteristics of different light sources. Turning it from a spectroscope to a spectrometer required a Raspberry Pi and a camera; mounted to a lens and positioned to see the spectrum created by the diffraction grating, the camera sends data to the Pi, where a Python program does the business of converting the spectrum to data. [Les]’s software is simple by complete, giving a graphical representation of the spectral data it sees. The video below shows the build process and what’s involved in calibrating the spectrometer, plus some of the more interesting spectra one can easily explore.

We appreciate the simplicity and the utility of this design, as well as its adaptability. Rather than using machined aluminum, the spectroscope holder and Pi cam bracket could easily be 3D-printer, and we could also see how the software could be adapted to use a PC and webcam.

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Interference Patterns Harnessed For Optical Logic Gates

The basics of digital logic are pretty easy to master, and figuring out how the ones and zeroes flow through various kinds of gates is often an interesting exercise. Taking things down a level and breaking the component AND, OR, and NOR gates down to their underlying analog circuits adds some complexity, but the flow of electrons is still pretty understandable. Substitute all that for photons, though, and you’ll enter a strange world indeed.

At least that’s our take on [Jeroen Vleggaar]’s latest project, which is making logic gates from purely optical components. As he himself admits in the video below, this isn’t exactly unexplored territory, but his method, which uses constructive and destructive interference, seems not to have been used before. The basic “circuit” consists of a generator, a pair of diffraction patterns etched into a quartz plate, and an evaluator, which is basically a pinhole in another plate positioned to coincide with the common focal point of the generator patterns. An OR gate is formed when the two generators are hit with in-phase monochromatic light. Making the two inputs out of phase by 180° results in an XOR gate, as destructive interference between the two inputs prevents any light from making it out of the evaluator.

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