It’s Printable, It’s Programmable, It’s E. Coli

Well, whaddya know? It seems that E. coli, the bane of Romaine and spinach everywhere, has at least one practical use. Researchers at Harvard have created a kind of 3D-printable ink that is alive and made entirely of microbes produced by E. coli. Although this is not the first so-called living ink, it does hold the title of the first living ink that doesn’t need any additional polymers to provide structure.

Passing the pillar test up to 16mm. Image via Nature

Because the ink is alive, it is technically programmable in the sense that it can self-assemble proteins into nanofibers, and further assemble those into nanofiber networks that comprise hydrogels.

One of the researchers compared the ink to a seed, which has everything it needs to eventually grow into a glorious tree. In this way, the ink could be used as a renewable building material both on Earth and in space. Though the ink does not continue to grow after being printed, the resulting structure would be a living system that could theoretically heal itself.

The ink creation process begins when the researchers induce genetically-engineered bacteria cultures to grow the ink, which is also made of living cells. The ink is then harvested and becomes gelatin-like, holding its shape well enough to go through a 3D printer. It even passes the bridging test, supporting its own weight between pillars placed up to 16 mm apart. (We’d like to see a Benchie.)

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3D Printing Gets Tiny

Using a process akin to electroplating, researchers at the University of Oldenburg have 3D printed structures at the 25 nanometer scale. A human hair, of course, is thousands of time thicker than that. The working medium was a copper salt and a very tiny nozzle. How tiny? As small as 1.6 nanometers. That’s big enough for two copper ions at once.

Tiny nozzles are prone to every 3D printer’s bane: clogged nozzles. To mitigate this, the team built a closed-loop control that measured electrical current between the work area and inside the nozzle. You can read the full paper online.

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Tiny 3D Printed Magnets Show Patterns

You normally associate a double helix with DNA, but an international team headquartered at Cambridge University used 3D printing to create magnetic double helixes that are about a 1,000 times smaller than a human hair. Why do such a thing? We aren’t sure why they started, but they were able to find nanoscale topological features in the magnetic field and they think it will change how magnetic devices work in the future — especially magnetic storage devices.

In particular, researchers feel this is a step towards practical “racetrack” memory that stores magnetic information in three dimensions instead of two and offer high density and RAM-like access times. You can read the full paper if you want the gory details.

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When Hacking And Biosensing Collide

[Prof. Edwin Hwu] of the Technical University of Denmark wrote in with a call for contributions to special edition of the open-access scientific journal Biosensors. Along the way, he linked in videos from three talks that he’s given on hacking consumer electronics gear for biosensing and nano-scale printing. Many of them focus on clever uses of the read-write head from a Blu-ray disc unit (but that’s not all!) and there are many good hacks here.

For instance, this video on using the optical pickup for the optics in an atomic force microscope (AFM) is bonkers. An AFM resolves features on the sub-micrometer level by putting a very sharp, very tiny probe on the end of a vibrating arm and scanning it over the surface in question. Deflections in the arm are measured by reflecting light off of it and measuring their variation, and that’s exactly what these optical pickups are designed to do. In addition to phenomenal resolution, [Dr. Hwu’s] AFM can be made on a shoestring budget!

Speaking of AFMs, check out his version that’s based on simple piezo discs in this video, but don’t neglect the rest of the hacks either. This one is a talk aimed at introducing scientists to consumer electronics hacking, so you’ll absolutely find yourself nodding your heads during the first few minutes. But then he documents turning a DVD player into a micro-strobe for high speed microfluidics microscopy using a wireless “spy camera” pen. And finally, [Dr. Hwu’s] lab has also done some really interesting work into nano-scale 3D printing, documented in this video, again using the humble Blu-ray drive, both for exposing the photopolymer and for spin-coating the disc with medium. Very clever!

If you’re doing any biosensing science hacking, be sure to let [Dr. Hwu] know. Or just tear into that Blu-ray drive that’s collecting dust in your closet.

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FlyBrainLab: Google Earth But For A Drosophila Fly’s Brain

In biology there are a couple of truly crucial model animals and insects. Not that they’re particularly good students, or pick up their own trash, but in the sense that they have become standard model organisms for research. Aside from genetic research, the FlyEM project seeks to fully map a little fly’s brain’s neural connections. This common fly, called drosophila melanogaster (or ‘lesser fruit fly’) has been the subject of a lot of genetic studies, but this study of its brain structure may provide insights in how our brain works as well.

Based on electron microscope images of thin slices of a drosophila brain, the three-dimensional structure of this tiny brain is reconstructed to not only determine the location of each neuron, but also their connections with other neurons. We know that about two-thirds of their brain is dedicated to processing the visual information from their relatively advanced compound eyes, but a lot is still unknown about how this is done, or how the brain’s structure develops.

If it’s always been your dream to tinker with a little fruit fly’s brain, you can do so yourself using the open source FlyBrainLab tool provided, along with the freely available data sources. This tool does not just allow one to visualize the drosophila brain in great detail, but also to create executable circuits and study their functionality. With neurobiology still a largely unexplored territory, this makes for an amazing tool to make this research accessible to anyone.

(Thanks for the tip, [Hernandi Krammes])

Laser Sees Through Keyhole

Those guys at Stanford must be watching a lot of James Bond movies. Their latest invention is a laser that can image an entire room through a keyhole. We imagine that will show up in a number of spy movies real soon now. You can see the code or watch the video below.

The technique is called NLOS or non-line-of-sight imaging. Previous approaches require scanning a large area to find indirect light from hidden objects. This new approach uses a laser to find objects that are moving. The indirect data changes based on the movement and an algorithm can reverse the measurements to determine the characteristics of the object.

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Screenshot from the video in question, showing 12:54 of the video, demonstrating how the electrons are being exchanged when circuit is completed

Li-ion Battery Low-Level Intricacies Explained Excellently

There’s a lot of magic in Lithium-ion batteries that we typically take for granted and don’t dig deeper into. Why is the typical full charge voltage 4.2 V and not the more convenient 5 V, why is CC/CV charging needed, and what’s up with all the fires? [The Limiting Factor] released a video that explains the low-level workings of Lithium-ion batteries in a very accessible way – specifically going into ion and electron ion exchange happening between the anode and the cathode, during both the charge and the discharge cycle. The video’s great illustrative power comes from an impressively sized investment of animation, script-writing and narration work – [The Limiting Factor] describes the effort as “16 months of animation design”, and this is no typical “whiteboard sketch” explainer video.

This is 16 minutes of pay-full-attention learning material that will have you glued to your screen, and the only reason it doesn’t explain every single thing about Lithium-ion batteries is because it’s that extensive of a topic, it would require a video series when done in a professional format like this. Instead, this is an excellent intro to help you build a core of solid understanding when it comes to Li-ion battery internals, elaborating on everything that’s relevant to the level being explored – be it the SEI layer and the organic additives, or the nitty-gritty of the ion and electron exchange specifics. We can’t help but hope that more videos like this one are coming soon (or as soon as they realistically can), expanding our understanding of all the other levels of a Li-ion battery cell.

Last video from [The Limiting Factor] was an 1-hour banger breaking down all the decisions made in a Tesla Battery Day presentation in similarly impressive level of detail, and we appreciate them making a general-purpose insight video – lately, it’s become clear we need to go more in-depth on such topics. This year, we’ve covered a great comparison between supercapacitors and batteries and suitable applications for each one of those, as well as explained the automakers’ reluctance to make their own battery cells. In 2020, we did a breakdown of alternate battery chemistries that aim to replace Li-ion in some of its important applications, so if this topic catches your attention, check those articles out, too!

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