On-Site Viral RNA Detection In Wastewater With Paper And Wax Microfluidics

October 9, 2024 by Maya Posch 4 Comments

Schematic version of on-site wastewater analysis using the microfluidic strips (Credit: Yuwei Pan et al., Cell, 2024)

Wastewater sampling has become a popular way over the years to keep track of the health of a population, including human ones, as pathogens are often detectable in the effluence from toilets. Since most houses connected to the centralized sewer systems, this means that a few sampling sites suffice to keep tabs on which viruses are circulating in an area. While sampling this wastewater is easy, the actual RNA analysis using PCR (polymerase chain reaction) still has to be performed in laboratories, adding complex logistics. An approach for on-site analysis using microfluidics was tested out by [Yuwei Pan] et al., as recently published in Cell.

This particular approach uses RT-LAMP (reverse-transcription loop-mediated isothermal amplification) to increase the amount of genetic material, which has the significant benefit over PCR that it does not require multiple thermal cycles, instead being run at a constant temperature. The filter paper used as the basis has wax microchannels printed on it, which help to guide the filtered wastewater to the reaction chambers. This is in many ways reminiscent of the all too familiar linear flow self-tests (RAT: rapid antigen test) that have become one of the hallmarks of the SARS-CoV-2 pandemic.

What this paper microfluidic device adds is that it doesn’t merely contain antigens, but performs the lysis (i.e. breakdown of the virus particles), genetic material multiplication using RT-LAMP and subsequent presence detection of certain RNA sequences to ascertain the presence of specific viruses. Having been used in the field already since 2020 in the UK, the researchers envision this type of on-site analysis to be combined with a smartphone for instant recording and transmission to health authorities.

Some of the benefits of this approach would be lower cost, easier logistics and faster results compared to shipping wastewater samples to central laboratories.

Electronic Covid Test Tear Down Shows Frustrating Example Of 1-Time-Use Waste

October 17, 2021 by Al Williams 68 Comments

The latest video from [TheSignalPath] is a result of his purchase of a home COVID-19 test. He found an electronic version that connects to your cell phone and displays the results on the phone. The device is an antigen test and, internally, works like the home tests that show the results using lines similar to a pregnancy test. So, somehow, the phone version reads the lines and communicates with the phone. But how? That’s the point of the video, which you can see below.

In a traditional test, there’s a control line that has to appear to show that the test was done correctly. Then a line under that indicates detection of the virus. The circuit board inside the electronic test has a plastic unit onboard that contains a similar strip and has optical sensors for both the reference line and the detection line. Since it is essentially an optical device — there are some lenses in the strip assembly that look like they are detecting the dye as it moves through the strip with LEDs onboard to shed light on the situation.

Under the microscope, the CPU is a typical Bluetooth-capable ARM chip from Nordic. The board did power up, but the device is made to only operate once because of the test strip. The video notes — and we agree — it seems wasteful to create an entire Bluetooth-enabled microcontroller board with optical components just to read a strip one time that is pretty easy to read to start with. We’ll stick with the simple test strip. Still, it is interesting to see the insides.

If you want to read more about antigen tests, we covered that. We also talked about PCR testing.

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A Stress Monitor Designed Specifically To Help You Work From Home

August 11, 2021 by Orlando Hoilett 10 Comments

There are quite a bit of mixed emotions regarding working from home. Some people love it and are thriving like they haven’t before, but others are having a bit of a hard time with it all. [Brandon] has been working from home for the last 12 years, but even after so many years of managing this type of work culture, he admits that it can still be a little stressful. He says he doesn’t take enough time in between tasks to simply relax and to breathe a little and the day-to-day minutia of his work can drive his stress level up if he doesn’t take some time to calm himself. He figured he could make something to monitor his stress level and remind himself to take a break and the results are pretty impressive.

He develops a system to monitor his heart rate and the ambient noise level in his room and uses these metrics as a measure of stress. If his heart rate or the ambient noise level goes above a certain threshold, then he sends himself a text message reminding himself to relax and take a break. You’ve probably seen people use heart rate as a measure of stress already, but you’re probably less familiar with using sound. [Brandon] basically thought the sound sensor would detect if he starts ranting for prolonged periods of time or if he’s in a Zoom meeting that gets too heated. We thought that was pretty neat.

[Brandon] used an off-the-shelf chest strap heart rate monitor to save himself a bit of time in trying to build his own. The device sends heart rate data to an nRF52840 over Bluetooth and then pushes the data to the cloud using a Blues Wireless Notecard. The Notecard also offers data encryption which gave [Brandon] some added peace of mind knowing his biometric data wasn’t floating around in the cloud without any sort of protection. This certainly isn’t medical-grade encryption, but it gave him a bit of comfort, nonetheless. All that data is processed in his custom-designed web app and when the appropriate thresholds are reached, he sends a text message to himself using Twilio reminding him to relax and unwind for a bit.

For his next iteration, [Brandon] might try making his own heart rate monitor. But until then, stay safe everybody, and remember to take a break whenever you need it.

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Distance Learning Land

September 16, 2020 by Orlando Hoilett 7 Comments

[familylovermommy] has been homeschooling her kids even before the pandemic, so she’s pretty well-versed on being a learning coach and a teacher. One of the activities she designed for her boys has them creating 3D models using Tinkercad. In the spirit of openness and cultivating freethinking, she did not give them very many constraints. But rather, gave them the liberty to creatively design whatever scene they imagined.

In the Instructable, she shares her sons’ designs along with instructions to recreate the models. The designs as you’ll see are pretty extensive, so she embedded the Tinkercad designs directly into it. You can even see a number of video showcases as well.

This is a really cool showcase of some pretty stellar workmanship. Also, maybe a bit of inspiration for some of our readers who are creating work from home activities of their own.

While you’re at it, check out some of these other work-from-home hacks.

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Fog-Free Mask Hack Solves Mask Versus Glasses Conundrum With Superb Seal

September 2, 2020 by Brian McEvoy 35 Comments

If you have worn a mask and glasses together for more than a quarter of a second, you are probably annoyed that we don’t have a magical solution for foggy lenses. Moisture-laden air is also a good indicator of where unfiltered air is escaping. Most masks have some flexible metal across the nose bridge that is supposed to seal the top, but it is woefully inadequate. The Badger Seal by [David Rothamer] and [Scott Sanders] from the University of Wisconsin-Madison College of Engineering is free to copy during the COVID-19 pandemic, even commercially. It works by running an elastic cord below the jaw and a formable wire over the nose to encourage contact all around both mouth and nose.

You can build your own in three ways. Each configuration is uniquely suited to a different situation. The first design is the easiest to make and should work for most people. The second is best for folks who need a better seal on the lower half of their face, like someone sporting a beard. It can also have ear loops, and that means your 3D printed ear savers have another use. The Madison campus of the University of Wisconsin also has fun with lock cracking and graphene experiments.
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Facing The Coronavirus

August 22, 2020 by Brian McEvoy 1 Comment

Some of us are oblivious to how often we touch our faces. The current finding is we reach for our eyes, nose, or mouth every three to four minutes. Twenty times per hour is an awful lot of poking, picking, itching, and prodding when we’re supposed to keep our hands away from glands that can transmit and receive disease. To curb this habit and enter the 2020 Hackaday Prize, [Lloyd lobo] built a proof-of-concept device that sounds the alarm when you reach for your face.

We see an Arduino Uno connected to the classic HC-SR04 ultrasonic distance sensor, an LED, and we have to assume a USB battery pack. [Lloyd] recommends the smaller Nano, we might reach for the postage-stamp models and swap the ultrasonic module out for the much smaller laser time of flight sensor. At its soul, this is an intruder alarm. Instead of keeping siblings out of your room, you will be keeping your hands out of the area below the bill of the hat where the sensor is mounted. If you regularly lift a coffee cup to your lips, it might chastise you, and if you chew sunflower seeds, you might establish a tempo. *crunch* *chip* *beep* *crunch* *chip* *beep*

We have reviewed technology to improve our habits like a bracelet that keeps a tally, and maybe there is a book that will help shirk some suboptimal behaviors.

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Hackaday Podcast 079: Wobble Sphere, Pixelflut, Skeeter Traps, And Tracing Apps

August 7, 2020 by Mike Szczys 3 Comments

Hackaday editors Mike Szczys and Elliot Williams gaze upon the most eye-popping projects from the past week. Who would have known that springy doorstops could be so artistic? Speaking of art, what happens if you give everyone on the network the chance to collectively paint using pixels? There as better way to catch a rat, and a dubious way to lure mosquitoes. We scratch our heads at sending code to the arctic, and Elliot takes a deep look at the contact tracing apps developed and in use throughout Europe.

Take a look at the links below if you want to follow along, and as always, tell us what you think about this episode in the comments!

Direct download (60 MB or so.)