Hacked Heating Instruments For The DIY Biology Lab

November 12, 2018 by Dan Maloney 4 Comments

[Justin] from The Thought Emporium takes on a common molecular biology problem with these homebrew heating instruments for the DIY biology lab.

The action at the molecular biology bench boils down to a few simple tasks: suck stuff, spit stuff, cool stuff, and heat stuff. Pipettes take care of the sucking and spitting, while ice buckets and refrigerators do the cooling. The heating, however, can be problematic; vessels of various sizes need to be accommodated at different, carefully controlled temperatures. It’s not uncommon to see dozens of different incubators, heat blocks, heat plates, and even walk-in environmental chambers in the typical lab, all acquired and maintained at great cost. It’s enough to discourage any would-be biohacker from starting a lab.

[Justin] knew It doesn’t need to be that way, though. So he tackled two common devices: the incubator and the heating block. The build used as many off-the-shelf components as possible, keeping costs down. The incubator is dead simple: an insulated plastic picnic cooler with a thermostatically controlled reptile heating pad. That proves to be more than serviceable up to 40°, at the high end of what most yeast and bacterial cultures require.

The heat block, used to heat small plastic reaction vessels called Eppendorf tubes, was a little more complicated to construct. Scrap heat sinks yielded aluminum stock, which despite going through a bit of a machinist’s nightmare on the drill press came out surprisingly nice. Heat for the block is provided by a commercial Peltier module and controller; it looks good up to 42°, a common temperature for heat-shocking yeast and tricking them into taking up foreign DNA.

We’re impressed with how cheaply [Justin] was able to throw together these instruments, and we’re looking forward to seeing how he utilizes them. He’s already biohacked himself, so seeing what happens to yeast and bacteria in his DIY lab should be interesting.

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Making Colored Smoke Devices, The Right Way

November 10, 2018 by Lewin Day 15 Comments

Pyrotechnics are fun, and, with the proper precautions taken, safe enough to play with at home (usually). While it’s typical to purchase fireworks and smoke devices off the shelf, it’s actually possible to brew these up in a properly stocked home lab. [Tech Ingredients] is here to share the techniques behind producing your own super vibrant colored smoke devices at home.

Producing colored smoke requires a slightly different tack than making a simpler white smoke device. Colored smokes use dyes that are temperature sensitive, and thus the reaction temperature must be controlled carefully. This is achieved by choosing a potassium chlorate oxidiser, and combining it with magnesium carbonate and sodium bicarbonate, which help stop the reaction getting too hot. Sugar is used as the primary fuel, with both lactose and sucrose being fit for purpose. Color is then added with solvent-based dyes, readily sourced online. These are stable at higher temperatures than typical water-based food grade dyes, and thus are the best choice for creating thick, vibrant colored smoke.

[Tech Ingredients] does a great job of explaining both the theory behind the work, as well as the practical considerations necessary to be successful. The video is the result of much experimentation and work off-camera, which shows in the final presentation. If you’ve been working on your own pyrotechnic creations, be sure to hit up the tips line. Video after the break.

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Arachnid Ale Uses Yeast To Make Spider Silk

November 5, 2018 by Brian McEvoy 21 Comments

Many people who read Hackaday hold the title of “Webmaster” but [The Thought Emporium] is after slightly different credentials with the same title. He aims to modify a strain of yeast to produce spider silk. Charlotte’s Web didn’t go into great detail about the different types of silk that a spider can produce, but the video and screencap after the break give a rundown of how spiders make different types of silk, and that each species of spider makes a unique silk. For this experiment, the desired silk is “beta sheets” which the video explains are hard and strong.

Some of the points mentioned in the video rely on things previously mentioned in other videos, but if you are the type of person excited by genetic modifications or using modified yeast to produce something made by another lifeform, you will probably be just fine. This is one of the most technical videos made by [The Thought Emporium] as he goes into the mechanisms of the modifications he will be making to the yeast. It sounds like a lot of work and the financial benefit of being able to produce spider silk affordably could be great, but in true hacker form, the procedure and results will be made freely available.

For some background into this hacker’s mind, check out how he has hacked his own lactose intolerance and even produced graphene through electrochemical exfoliation.

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Failing At Making Ferrofluid

October 26, 2018 by Al Williams 17 Comments

[NileRed] admits that while ferrofluid has practical uses, he simply wanted to play with it and didn’t want to pay the high prices he found in Canada. A lot of the instructions he found were not for making a true ferrofluid. He set out to create the real thing, but he wasn’t entirely successful. You can see the results — which aren’t bad at all — in the video below.

We’ve always said you learn more from failure than success. The process of creating ferrofluid involves two key steps: creating coated nanoparticles of magnetite and removing particles that are too large or improperly coated. After the first not entirely satisfactory attempt, [NileRed] tried to purify the material using solvents and magnets to create better-quality particles. Even the “bad” material, though, looked fun to play with along with a powerful magnet.

You’ll see that the material is clearly magnetic, it just doesn’t spike like normal ferrofluid. [NileRed] had commercial ferrofluid for testing and found that if he diluted it enough, it behaved like his homemade fluid. So while not conclusive, it seems like he diluted the batch too much.

We hope to see a better batch from him soon. The base material he used for the first patch was homemade — he covers that in a different video. However, for the second batch, he is going to start with commercial ferric chloride — what we know as PCB etchant.

Even though the experiment was not entirely successful, we enjoyed seeing the process and watching the performance of both the homemade batch and the commercial ferrofluid. He’s getting a lot of advice and speculation in the video comments, and it is very possible a Hackaday reader might be able to help, too.

We’ve seen other reports of unsuccessful ferrofluid production. If you need a practical reason to make or buy some, how about a clock?

Retrotechtacular: Disposing Of Sodium, 1947-Style

October 3, 2018 by Dan Maloney 55 Comments

A high school friend once related the story about how his father, a chemist for an environmental waste concern, disposed of a problematic quantity of metallic sodium by dumping it into one of the more polluted rivers in southern New England. Despite the fact that the local residents were used to seeing all manner of noxious hijinx in the river, the resulting explosion was supposedly enough to warrant a call to the police and an expeditious retreat back to the labs. It was a good story, but not especially believable back in the day.

After seeing this video of how the War Department dealt with surplus sodium in 1947, I’m not so sure. I had always known how reactive sodium is, ever since demonstrations in chemistry class where a flake of the soft gray metal would dance about in a petri dish full of water and eventually light up for a few exciting seconds. The way the US government decided to dispose of 20 tons of sodium was another thing altogether. The metal was surplus war production, probably used in incendiary bombs and in the production of aluminum for airplanes. No longer willing to stockpile it, the government tried to interest industry in the metal, but to no avail due to the hazard and expense of shipping the stuff. Sadly (and as was often the case in those days), they just decided to dump it.

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Testing Lithium Ceramic Batteries (LCBs)

September 15, 2018 by Steven Dufresne 29 Comments

Affordable solid-state batteries large enough for cell phones and drones have been promised for a long time but seem to always be a few years away from production. In this case, Taiwan based Prologium sent [GreatScott] samples of their Lithium Ceramic batteries (LCBs) to test, and even though they’re not yet commercial products, who are we to refuse a peek at what they’ve been up to? They sent him two types, flexible ones (FLCBs) and higher capacity stiff ones (PLCBs).

The FLCBs were rated at 100 mAh and just 2 C, both small values but still useful for wearables, especially given their flexibility. Doing some destructive testing, he managed to keep an LED lit while flexing the battery and cutting away at it with tin snips.

Switching to the thicker 7.31 Wh PLCB, he measured and weighed it to get an energy density of 258 Wh/L and a specific energy of 118 Wh/kg, only about 2/3rds and 1/2 that of his LiPo and lithium-ion batteries. Repeating the destructive tests with these ones, the LED turned off and smoke appeared while cutting and hammering a nail through, likely due to the shorts caused by the electrically conductive tin snips and nail. But once the snips and nail were moved away, the smoke stopped and the LED lit up again. Overcharging and short-circuiting the batteries both caused the solder connecting the wires to them to melt but nothing else happened. Rapidly discharging through a resistor only resulted in a gradual voltage drop. Clearly, these batteries are much safer than their LiPo and lithium ion counterparts. That safety and their flexibility seem to be their current main selling points should they become available for us hackers. Check out his tests in the video below.

Meanwhile, we’ll have to be content with the occasional tantalizing report from the labs such as this one from MIT of a long battery life and another from one of the co-inventors of the lithium-ion battery which uses a glass electrolyte.

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Chemistry And Lasers Turn Any Plastic Surface Into A PCB

September 4, 2018 by Dan Maloney 30 Comments

On the face of it, PCB production seems to pretty much have been reduced to practice. Hobbyists have been etching their own boards forever, and the custom PCB fabrication market is rich with vendors whose capabilities span the gamut from dead simple one-side through-hole boards to the finest pitch multilayer SMD boards imaginable.

So why on Earth would we need yet another way to make PCBs? Because as [Ben Krasnow] points out, the ability to turn almost any plastic surface into a PCB can be really handy, and is not necessarily something the fab houses handle right now. The video below shows how [Ben] came up with his method, which went down a non-obvious path that was part chemistry experiment, part materials science. The basic idea is to use electroless copper plating, a method of depositing copper onto a substrate without using electrolysis.

This allows non-conductive substrates — [Ben] used small parts printed with a Formlabs SLA printer — to be plated with enough copper to form solderable traces. The chemistry involved in this is not trivial; there are catalysts and surfactants and saturated solutions of copper sulfate to manage. And even once he dialed that in, he had to figure out how to make traces and vias with a laser cutter. It was eventually successful, but it took a lot of work. Check out the video below to see how he got there, and where he plans to go next.

You’ve got to hand it to [Ben]; when he decides to explore something, he goes all in. We appreciate his dedication, whether he’s using candles to explore magnetohydrodynamics or making plasma with a high-speed jet of water.

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