Biology Lab On Your Christmas List

December 11, 2018 by Brian McEvoy 6 Comments

We hope you have been good this year because we have a list to start your own biology lab and not everything will fit into Santa’s bag (of holding). If you need some last minute goodie points, Santa loves open-source and people who share on our tip line. Our friends at [The Thought Emporium] have compiled a list of the necessary equipment for a biology lab. Chemistry labs-in-a-box have been the inspiration for many young chemists, but there are remarkable differences between a chemistry lab and a biology lab which are explained in the Youtube video linked above and embedded after the break.

If you are preparing to start a laboratory or wondering what to add to your fledging lab, this video is perfect. It comes from the perspective of a hacker not afraid to make tools like his heat block and incubator which should absolutely be built rather than purchased but certain things, like a centrifuge, should be purchased when the lab is mature. In the middle we have the autoclave where a used pressure cooker may do the trick or you may need a full-blown commercial model with lots of space and a high-pressure range.

Maybe this will take some of the mystique out of starting your own lab and help you understand what is happening with a gel dock or why a spectrophotometer is the bee’s knees. There are a handful of other tools not mentioned here so if this is resonating, it will be worth a watch.

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Simple, Low-Cost Rig Lets The Budding Biohacker Run DNA Gels

December 1, 2018 by Dan Maloney 12 Comments

We all the know the basic components for building out an electronics lab: breadboards, bench power supply, a selection of components, a multimeter, and maybe an oscilloscope. But what exactly do you need when you’re setting up a biohacking lab?

That’s the question that [Justin] from The Thought Emporium is trying to answer with a series of videos where he does exactly that – build a molecular biology lab from scratch. In the current installment, [Justin] covers the basics of agarose gel electrophoresis, arguably the fundamental skill for aspiring bio-geeks. Electrophoresis is simply using an electric field to separate a population of macromolecules, like nucleic acids and proteins, based on their sizes. [Justin] covers the basics, from building a rig for running agarose gels to pouring the gels to doing the actual separation and documenting the results. Commercial grade gear for the job is priced to squeeze the most money out of a grant as possible, but his stuff is built on the cheap, from dollar-store drawer organizers and other odd bits. It all works, and it saves a ton of money that can be put into the things that make more sense to buy, like fluorescent DNA stain for visualizing the bands; we’re heartened to see that the potent carcinogen ethidium bromide that we used back in the day is no longer used for this.

We’re really intrigued with [Justin]’s bio lab buildout, and it inspires us to do the same here. This and other videos in the series, like his small incubators built on the cheap, will go a long way to helping others get into biohacking.

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Video Details Construction Of Transparent Wood

November 27, 2018 by Al Williams 11 Comments

We’ve talked about transparent wood before. However, the process can be difficult to get just right. [NileRed] recently posted a video with very detailed instructions on how he’s doing it. Aside from the dangerous way he uses a table saw — something he realized after he watched the video — it is some great information.

This isn’t some hand-waving explanation. For nearly 36 minutes, you get an actual demonstration of the steps along with some explanations about why it works and why certain steps are done in a particular way.

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Solar-Powered IoT Sensor Saves Wine Batch From Overheating

November 25, 2018 by Donald Papp 4 Comments

Making wine isn’t just about following a recipe, it’s a chemical process that needs to be monitored and managed for best results. The larger the batch, the more painful it is to have something go wrong. This means that the stakes are high for small vineyards such as the family one [Mare] works with, which have insufficient resources to afford high-end equipment yet have the same needs as larger winemakers. The most useful thing to monitor is the temperature profile of the fermentation process, and [Mare] created an exceptional IoT system to do that using LoRa wireless and solar power.

It’s not enough just to measure temperature of the fermenting liquid; viewing how the temperature changes over time is critical to understanding the process and spotting any trouble. [Mare] originally used a Raspberry Pi, I2C temperature sensor, and a Wi-Fi connection to a database to do the monitoring. This was a success, but it was also overkill. To improve the system, the Raspberry Pi was replaced with a LoRaDunchy board, an STM-based module of [Mare]’s own design which is pin-compatible with the Arduino Nano. It includes a battery charger, power management, and LoRa wireless communication. Adding a solar cell and lithium-polymer battery was all it took to figuratively cut the power cord.

Sensing the temperature of fermentation is done by sealing the temperature sensor into a thin aluminum tube, and lowering that into the vat. There it remains, with the LoRaDunchy board periodically waking up to read the sensor and report the tempurature over LoRa before going back to sleep, all the while sipping power from the battery which in turn gets recharged with solar power.

It’s an elegant system that has already paid off. A 500 litre vat of wine generated an alarm when the temperature rose above 24 Celsius for 10 minutes. An email alert allowed the owner to begin mixing the solution and add ice water to put the brakes on the runaway reaction. The temperature dropped and slow fermentation resumed, thanks to the twin powers of gathering the right data, then doing something meaningful with it.

Vineyards and LoRa have joined forces before, for example in the Vinduino project which aims to enable water-smart farming. If you’re unfamiliar with LoRa in general, the LoRa on the ESP32 project page contains a good primer, and if the antenna on the module shown here looks familiar to you it’s because we recently featured [Mare]’s guide on making DIY LoRa antennas from salvaged wire.

Electrolysis Tank Removes Rust

November 24, 2018 by Al Williams 18 Comments

If you have something rusty, you can get a wire brush and a lot of elbow grease. Or you can let electricity do the work for you in an electrolysis tank. [Miller’s Planet] shows you how to build such a tank, but even better, he explains why it works in a very detailed way.

The tank uses a sodium carbonate electrolyte — just water and washing powder. In the reaction, free electrons from the electrolyte displace the oxygen from the rusted metal piece. A glass container, a steel rod, and a power supply make up the rest.

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Etch Your Own Circuit Boards In Your Kitchen

November 16, 2018 by Brian Benchoff 43 Comments

Right now, you can design a PCB, send it off to a PCB fab, and get professional finished boards in a few days for less than a dollar per square inch. This is fantastic, and it’s the driving force behind ever-dropping costs of hardware development. That’s great and all, but you can make circuit boards at home, easily, and without involving too many toxic chemicals. That’s exactly what [videoschmideo] did, and the results are pretty good.

The process starts with a single-sided copper clad board that would be readily obtainable at Radio Shack if there were any of those around anymore. Once the circuit is designed, the traces and pads are printed (mirrored) out onto sticker backing paper. The toner from your laser printer is transferred to the copper with a clothes iron.

The tricky part about creating a PCB is taking away all the copper you don’t want, and for this tutorial [videoschmideo] is using a vinegar and hydrogen peroxide process. If you’re using stuff you can buy at the grocery store, you’re only getting 3% acetic acid and 3% peroxide, but given enough time and enough peroxide, it’ll do the job. After the board is etched, [videoschmideo] neutralizes the copper acetate produced with aluminum foil. The end product isn’t the safest thing in the world, but aluminum salts are much more environmentally friendly than copper compounds.

Making PCBs at home isn’t anything new, but it’s nice to be reminded that you can do so even with minimal effort and chemicals that you could rinse your mouth with. Once you do, though, you’ll probably have to drill some holes in the board. Yes, you could use a dremel, but a nice small drill press is a pleasure, and well worth the investment.

Measuring The Cooling Effect Of Transformer Oil

November 15, 2018 by Steven Dufresne 36 Comments

Transformer oil has long served two purposes, cooling and insulating. The large, steel encased transformers we see connected to the electrical grid are filled with transformer oil which is circulated through radiator fins for dumping heat to the surrounding air. In the hacker world, we use transformer oil for cooling RF dummy loads and insulating high voltage components. [GreatScott] decided to do some tests of his own to see just how good it is for cooling circuits.

He started with testing canola oil but found that it breaks down from contact with air and becomes rancid. So he purchased some transformer oil. First, testing its suitability for submerging circuits, he found that he couldn’t see any current above his meter’s 0.0 μA limit when applying 15 V no matter how close together he brought his contacts. At 1 cm he got around 2 μA with 230 VAC, likely from parasitic capacitance, for a resistance of 115 Mohm/cm.

Moving on to thermal testing, he purchased a 4.7 ohm, 100 watt, heatsink encased resistor and attached a temperature probe to it with Kapton tape. Submerging it in transformer oil and applying 25 watts through it continuously, he measured a temperature of 46.8°C after seven minutes. The same test with distilled water reached 35.3°C. Water’s heat capacity is 4187 J/kg∙K, not surprisingly much better than the transformer oil’s 2090 J/kg∙K which in turn is twice as good as air’s 1005 J/kg∙K.

He performed a few more experiments but we’ll leave those to his video below.

We’ve run across a number of tests running boards submerged in various oils before. For example, we’ve seen Raspberry Pi’s running in vegetable oil and mineral oil as well as an Arduino running in a non-conductive liquid coolant, all either overclocked or under heavy load.

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