Fluorescence Microscope On A Hacker’s Budget

March 30, 2019 by Brian McEvoy 19 Comments

Some of biology’s most visually striking images come from fluorescence microscopes. Their brilliant colors on black look like a neon sign from an empty highway. A brand new fluorescence microscope is beyond a hacker’s budget and even beyond some labs’, but there are ways to upgrade an entry-level scope for the cost of a few cups of coffee. [Justin Atkin] of The Thought Emporium published a scope hacking video which can also be seen below. He is becoming a reputed scope modder.

This video assumes a couple of things for the $10 price tag. The first premise is that you already have a scope, a camera adapter, and a camera capable of shooting long exposures. The second premise is that you are willing to break the seals and open the scope to make some reversible mods. Since you are reading Hackaday, maybe that is a given.

The premise is simple compared to the build, which is not rocket surgery, the light source from below illuminates the subject like a raver, and the filter removes any light that isn’t spectacular before it gets to the camera.

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Hackaday Podcast 007: Everything Microcontrollers, Deadly Clock Accuracy, CT X-Rays, Mountains Of E-Waste

February 22, 2019 by Mike Szczys 5 Comments

Elliot Williams and Mike Szczys look at all that’s happening in hackerdom. This week we dive deep into super-accurate clock chips, SPI and microcontroller trickery, a new (and cheap) part on the microcontroller block, touch-sensitive cloth, and taking a home X-ray to the third dimension. We’re saying our goodbyes to the magnificent A380, looking with skepticism on the V2V tech known as DSRC, and also trying to predict weather with automotive data. And finally, what’s the deal with that growing problem of electronic waste?

Links for all discussed on the show are found below. As always, join in the comments below as we’ll be watching those as we work on next week’s episode!

Take a look at the links below if you want to follow along, and as always, tell us what you think about this episode in the comments!

Direct download (60 MB or so.)

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MIT IAP Tackles Radio

February 18, 2019 by Al Williams 2 Comments

MIT is well known for rigorous courses, but they also have a special four-week term at the start of each year called the IAP — Independent Activities Period. This year, the MIT Radio Society had several interesting presentations on both the history and application of radio. You weren’t there? No problem, as the nine lecture were all recorded for you to watch at your leisure. You can see one of the nine, below.

These aren’t some five minute quicky videos, either. They are basically live captures that run anywhere from an hour to almost two hours in length. The topics are a great mix including radio history, software-defined radio, propagation, radio astronomy, RADAR, and even 5G.

You might have to pick and choose. Some of the lectures are suitable for just about anyone. Some assume a bit more radio expertise in electronics or math. Still, they are all worth at least a cursory skim to see if you want to really sit and watch in detail. The only nitpick is that some presenters used a laser pointer that doesn’t show up on the inset slide graphics in the video. That makes sense because the inset slides are not really in the room, but it can make it a little difficult to understand what the speaker is pointing to on a crowded slide.

Of course, if you want to dive deep and you need more background, MIT — along with many other institutions — will let you use their learning material for free. We were especially fans of the circuits class but there are many others including just raw materials from OCW.

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Spectrometer Is Inexpensive And Capable

February 9, 2019 by Brian McEvoy 14 Comments

We know the effect of passing white light through a prism and seeing the color spectrum that comes out of the other side. It will not be noticeable to the naked eye, but that rainbow does not fully span the range of [Roy G. Biv]. There are narrowly absent colors which blur together, and those missing portions are a fingerprint of the matter the white light is passing through or bouncing off. Those with a keen eye will recognize that we are talking about spectrophotometry which is identifying those fingerprints and determining what is being observed and how much is under observation. The device which does this is called a spectrometer and [Justin Atkin] invites us along for his build. Video can also be seen below.

Along with the build, we learn how spectrophotometry works, starting with how photons are generated and why gaps appear in the color spectrum. It is all about electrons, which some of our seasoned spectrometer users already know. The build uses a wooden NanoDrop style case cut on a laser engraver. It needs some improvements which are mentioned and shown in the video so you will want to have some aluminum tape on hand. The rest of the bill of materials is covered including “Black 2.0” which claims to be the “mattest, flattest, black acrylic paint.” Maybe that will come in handy for other optical projects. It might be wise to buy first surface mirrors cut to size, but you can always make bespoke mirrors with carefully chosen tools.

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Robot’s Actions And Our Reactions

January 12, 2019 by Brian McEvoy 9 Comments

If you walk into a dog owner’s home that dog is probably going to make a beeline to see if you are a threat. If you walk into a cat owner’s home, you may see the cat wandering around, if it even chooses to grace you with its presence. For some people, a dog’s direct approach can be nerve-wracking, or even scary depending on their history and relative size of the dog. Still, these domestic animals are easy to empathize with especially if you or your family have a pet. They have faces which can convey curiosity or smug indifference but what if you were asked to judge the intent of something with no analogs to our own physical features like a face or limbs? That is what researchers at the IDC Herzliya in Israel and Cornell University in the US asked when they made the Greeting Machine to move a moon-like sphere around a planet-like sphere.

Participants were asked to gauge their feelings about the robot after watching the robot move in different patterns. It turns out that something as simple as a sphere tracing across the surface of another sphere can stir consistent and predictable emotions in people even though the shapes do not resemble a human, domestic pet, or anything but a snowman’s abdomen. This makes us think about how our own robots must be perceived by people who are not mired in circuits all day. Certainly, a robot jellyfish lazing about in the Atlantic must feel less threatening than a laser pointer with a taste for human eyeballs.

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Trashed TV Gets RGB LED Backlight

October 11, 2018 by Tom Nardi 34 Comments

It might not be obvious unless you’ve taken one apart, but most of the TVs and monitors listed as “LED” are simply LCD panels that use a bank of LEDs to illuminate them from behind. Similarly, what are generally referred to as “LCDs” are LCD panels that use fluorescent tubes for illumination. To get a true LED display with no separate backlight, you need OLED. Confused? Welcome to the world of consumer technology.

With those distinctions in mind, the hack that [Zenodilodon] recently performed on a broken “LED TV” is really rather brilliant. By removing the dead white LED backlights and replacing them with RGB LED strips, he not only got the TV working again, but also imbued it with color changing abilities. Perfect for displaying music visualizations, or kicking your next film night into high gear with a really trippy showing of Seven Samurai.

In the video after the break, [Zenodilodon] starts his RGB transplant by stripping the TV down to its principal parts. The original LEDs were toasted, so they might as well go straight in the bin alongside their driver electronics. But the LCD panel itself was working fine (tested by shining a laser pointer through it to see if there was an image), and the plastic sheets which diffuse the LED backlight were easily salvaged.

With the old LEDs removed, [Zenodilodon] laid out his new strips and soldered them up to the external controller. He was careful to use all white wires, as he was worried colored wires might reflect the white light and be noticeable on the display. After buttoning the TV back up, he went through a few demonstrations to show how the image looked with the white LEDs on, as well as some interesting effects that could be seen when the LEDs are cycling through colors.

The RGB strips don’t light up the display as well as the original backlight did, as there are some obvious dark spots and you can see some horizontal lines where the strips are. But [Zenodilodon] says the effect isn’t too bad in real-life, and considering it was a cheap TV the image quality was probably never that great to begin with.

On the flip side, if you find an LED TV or monitor in the trash with a cracked screen, it might be worth taking it home to salvage its super-bright white LEDs for your lighting projects.

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Help With Stuttering Could Come From Electricity

July 24, 2018 by Brian McEvoy 10 Comments

At the University of Oxford, [Jen Chesters] conducts therapy sessions with thirty men in a randomized clinical trial to test the effects of tDCS on subjects who stutter. Men are approximately four times as likely to stutter and the sex variability of the phenomenon is not being tested. In the randomized sessions, the men and [Jen] are unaware if any current is being applied, or a decoy buzzer is used.

Transcranial Direct Current, tDCS, applies a small current to the brain with the intent of exciting or biasing the region below the electrode. A credit-card sized card is used to apply the current. Typically, tDCS ranges from nine to eighteen volts at two milliamps or less. The power passing through a person’s brain is roughly on par with the kind of laser pointer you should not point straight into your eyeball and is considered “safe,” with quotation marks.

A week after the therapy, conversational fluency and the ability to recite written passages shows improvement over the placebo group which does not show improvement. Six weeks after the therapy, there is still measurable improvement in the ability to read written passages, but sadly, conversational gains are lost.

Many people are on the fence about tDCS and we urge our citizen scientists to exercise all the caution you would expect when sending current through the brain. Or, just don’t do that.